A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress

We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the...

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Detalles Bibliográficos
Autores principales: Forcat, Silvia, Bennett, Mark H, Mansfield, John W, Grant, Murray R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504475/
https://www.ncbi.nlm.nih.gov/pubmed/18590529
http://dx.doi.org/10.1186/1746-4811-4-16
Descripción
Sumario:We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.

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