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Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin

BACKGROUND: The aim of the study was to detect whether in experimental Salmonella enterica Typhimurium infection the probiotic Lactobacillus fermentum ME-3 in combination with fluoroquinolone therapy would eradicate S. Typhimurium, prevent the development of liver and spleen granulomas and improve t...

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Autores principales: Truusalu, Kai, Mikelsaar, Raik-Hiio, Naaber, Paul, Karki, Tõnis, Kullisaar, Tiiu, Zilmer, Mihkel, Mikelsaar, Marika
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518159/
https://www.ncbi.nlm.nih.gov/pubmed/18680567
http://dx.doi.org/10.1186/1471-2180-8-132
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author Truusalu, Kai
Mikelsaar, Raik-Hiio
Naaber, Paul
Karki, Tõnis
Kullisaar, Tiiu
Zilmer, Mihkel
Mikelsaar, Marika
author_facet Truusalu, Kai
Mikelsaar, Raik-Hiio
Naaber, Paul
Karki, Tõnis
Kullisaar, Tiiu
Zilmer, Mihkel
Mikelsaar, Marika
author_sort Truusalu, Kai
collection PubMed
description BACKGROUND: The aim of the study was to detect whether in experimental Salmonella enterica Typhimurium infection the probiotic Lactobacillus fermentum ME-3 in combination with fluoroquinolone therapy would eradicate S. Typhimurium, prevent the development of liver and spleen granulomas and improve the indices of oxidative stress in the ileum mucosa. The selected bacteriological, histological and biochemical methods were applied. RESULTS: Combined treatment with L. fermentum ME-3 and ofloxacin eradicated Salmonella Typhimurium from blood, ileum and liver, decreased the number of animals with liver and spleen granulomas and reduced the value of lipid peroxides in the ileum mucosa. Higher total counts of intestinal lactobacilli in all experimental groups were associated with the absence of liver granulomas. CONCLUSION: The antimicrobial and antioxidative probiotic L. fermentum ME-3 combined with ofloxacin enhances the eradication of experimental S. Typhimurium infection. These observations on probiotic and antimicrobial co-action may serve as basis to develop new strategies for treatment of invasive bacterial infections of the gut.
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spelling pubmed-25181592008-08-20 Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin Truusalu, Kai Mikelsaar, Raik-Hiio Naaber, Paul Karki, Tõnis Kullisaar, Tiiu Zilmer, Mihkel Mikelsaar, Marika BMC Microbiol Research Article BACKGROUND: The aim of the study was to detect whether in experimental Salmonella enterica Typhimurium infection the probiotic Lactobacillus fermentum ME-3 in combination with fluoroquinolone therapy would eradicate S. Typhimurium, prevent the development of liver and spleen granulomas and improve the indices of oxidative stress in the ileum mucosa. The selected bacteriological, histological and biochemical methods were applied. RESULTS: Combined treatment with L. fermentum ME-3 and ofloxacin eradicated Salmonella Typhimurium from blood, ileum and liver, decreased the number of animals with liver and spleen granulomas and reduced the value of lipid peroxides in the ileum mucosa. Higher total counts of intestinal lactobacilli in all experimental groups were associated with the absence of liver granulomas. CONCLUSION: The antimicrobial and antioxidative probiotic L. fermentum ME-3 combined with ofloxacin enhances the eradication of experimental S. Typhimurium infection. These observations on probiotic and antimicrobial co-action may serve as basis to develop new strategies for treatment of invasive bacterial infections of the gut. BioMed Central 2008-08-04 /pmc/articles/PMC2518159/ /pubmed/18680567 http://dx.doi.org/10.1186/1471-2180-8-132 Text en Copyright © 2008 Truusalu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Truusalu, Kai
Mikelsaar, Raik-Hiio
Naaber, Paul
Karki, Tõnis
Kullisaar, Tiiu
Zilmer, Mihkel
Mikelsaar, Marika
Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title_full Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title_fullStr Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title_full_unstemmed Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title_short Eradication of Salmonella Typhimurium infection in a murine model of typhoid fever with the combination of probiotic Lactobacillus fermentum ME-3 and ofloxacin
title_sort eradication of salmonella typhimurium infection in a murine model of typhoid fever with the combination of probiotic lactobacillus fermentum me-3 and ofloxacin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518159/
https://www.ncbi.nlm.nih.gov/pubmed/18680567
http://dx.doi.org/10.1186/1471-2180-8-132
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