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Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle

Continuous poleward movement of tubulin is a hallmark of metaphase spindle dynamics in higher eukaryotic cells and is essential for stable spindle architecture and reliable chromosome segregation. We use quantitative fluorescent speckle microscopy to map with high resolution the spatial organization...

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Detalles Bibliográficos
Autores principales: Yang, Ge, Cameron, Lisa A., Maddox, Paul S., Salmon, Edward D., Danuser, Gaudenz
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518697/
https://www.ncbi.nlm.nih.gov/pubmed/18710922
http://dx.doi.org/10.1083/jcb.200801105
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author Yang, Ge
Cameron, Lisa A.
Maddox, Paul S.
Salmon, Edward D.
Danuser, Gaudenz
author_facet Yang, Ge
Cameron, Lisa A.
Maddox, Paul S.
Salmon, Edward D.
Danuser, Gaudenz
author_sort Yang, Ge
collection PubMed
description Continuous poleward movement of tubulin is a hallmark of metaphase spindle dynamics in higher eukaryotic cells and is essential for stable spindle architecture and reliable chromosome segregation. We use quantitative fluorescent speckle microscopy to map with high resolution the spatial organization of microtubule flux in Xenopus laevis egg extract meiotic spindles. We find that the flux velocity decreases near spindle poles by ∼20%. The regional variation is independent of functional kinetochores and centrosomes and is suppressed by inhibition of dynein/dynactin, kinesin-5, or both. Statistical analysis reveals that tubulin flows in two distinct velocity modes. We propose an association of these modes with two architecturally distinct yet spatially overlapping and dynamically cross-linked arrays of microtubules: focused polar microtubule arrays of a uniform polarity and slower flux velocities are interconnected by a dense barrel-like microtubule array of antiparallel polarities and faster flux velocities.
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spelling pubmed-25186972009-02-25 Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle Yang, Ge Cameron, Lisa A. Maddox, Paul S. Salmon, Edward D. Danuser, Gaudenz J Cell Biol Research Articles Continuous poleward movement of tubulin is a hallmark of metaphase spindle dynamics in higher eukaryotic cells and is essential for stable spindle architecture and reliable chromosome segregation. We use quantitative fluorescent speckle microscopy to map with high resolution the spatial organization of microtubule flux in Xenopus laevis egg extract meiotic spindles. We find that the flux velocity decreases near spindle poles by ∼20%. The regional variation is independent of functional kinetochores and centrosomes and is suppressed by inhibition of dynein/dynactin, kinesin-5, or both. Statistical analysis reveals that tubulin flows in two distinct velocity modes. We propose an association of these modes with two architecturally distinct yet spatially overlapping and dynamically cross-linked arrays of microtubules: focused polar microtubule arrays of a uniform polarity and slower flux velocities are interconnected by a dense barrel-like microtubule array of antiparallel polarities and faster flux velocities. The Rockefeller University Press 2008-08-25 /pmc/articles/PMC2518697/ /pubmed/18710922 http://dx.doi.org/10.1083/jcb.200801105 Text en © 2008 Yang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Yang, Ge
Cameron, Lisa A.
Maddox, Paul S.
Salmon, Edward D.
Danuser, Gaudenz
Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title_full Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title_fullStr Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title_full_unstemmed Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title_short Regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
title_sort regional variation of microtubule flux reveals microtubule organization in the metaphase meiotic spindle
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518697/
https://www.ncbi.nlm.nih.gov/pubmed/18710922
http://dx.doi.org/10.1083/jcb.200801105
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