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Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum

Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and occurs near the endoplasmic reticulum (ER), but the exact mechanisms are unknown. We show that double FYV...

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Detalles Bibliográficos
Autores principales: Axe, Elizabeth L., Walker, Simon A., Manifava, Maria, Chandra, Priya, Roderick, H. Llewelyn, Habermann, Anja, Griffiths, Gareth, Ktistakis, Nicholas T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518708/
https://www.ncbi.nlm.nih.gov/pubmed/18725538
http://dx.doi.org/10.1083/jcb.200803137
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author Axe, Elizabeth L.
Walker, Simon A.
Manifava, Maria
Chandra, Priya
Roderick, H. Llewelyn
Habermann, Anja
Griffiths, Gareth
Ktistakis, Nicholas T.
author_facet Axe, Elizabeth L.
Walker, Simon A.
Manifava, Maria
Chandra, Priya
Roderick, H. Llewelyn
Habermann, Anja
Griffiths, Gareth
Ktistakis, Nicholas T.
author_sort Axe, Elizabeth L.
collection PubMed
description Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and occurs near the endoplasmic reticulum (ER), but the exact mechanisms are unknown. We show that double FYVE domain–containing protein 1, a PI(3)P-binding protein with unusual localization on ER and Golgi membranes, translocates in response to amino acid starvation to a punctate compartment partially colocalized with autophagosomal proteins. Translocation is dependent on Vps34 and beclin function. Other PI(3)P-binding probes targeted to the ER show the same starvation-induced translocation that is dependent on PI(3)P formation and recognition. Live imaging experiments show that this punctate compartment forms near Vps34-containing vesicles, is in dynamic equilibrium with the ER, and provides a membrane platform for accumulation of autophagosomal proteins, expansion of autophagosomal membranes, and emergence of fully formed autophagosomes. This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation.
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spelling pubmed-25187082009-02-25 Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum Axe, Elizabeth L. Walker, Simon A. Manifava, Maria Chandra, Priya Roderick, H. Llewelyn Habermann, Anja Griffiths, Gareth Ktistakis, Nicholas T. J Cell Biol Research Articles Autophagy is the engulfment of cytosol and organelles by double-membrane vesicles termed autophagosomes. Autophagosome formation is known to require phosphatidylinositol 3-phosphate (PI(3)P) and occurs near the endoplasmic reticulum (ER), but the exact mechanisms are unknown. We show that double FYVE domain–containing protein 1, a PI(3)P-binding protein with unusual localization on ER and Golgi membranes, translocates in response to amino acid starvation to a punctate compartment partially colocalized with autophagosomal proteins. Translocation is dependent on Vps34 and beclin function. Other PI(3)P-binding probes targeted to the ER show the same starvation-induced translocation that is dependent on PI(3)P formation and recognition. Live imaging experiments show that this punctate compartment forms near Vps34-containing vesicles, is in dynamic equilibrium with the ER, and provides a membrane platform for accumulation of autophagosomal proteins, expansion of autophagosomal membranes, and emergence of fully formed autophagosomes. This PI(3)P-enriched compartment may be involved in autophagosome biogenesis. Its dynamic relationship with the ER is consistent with the idea that the ER may provide important components for autophagosome formation. The Rockefeller University Press 2008-08-25 /pmc/articles/PMC2518708/ /pubmed/18725538 http://dx.doi.org/10.1083/jcb.200803137 Text en © 2008 Axe et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Axe, Elizabeth L.
Walker, Simon A.
Manifava, Maria
Chandra, Priya
Roderick, H. Llewelyn
Habermann, Anja
Griffiths, Gareth
Ktistakis, Nicholas T.
Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title_full Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title_fullStr Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title_full_unstemmed Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title_short Autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
title_sort autophagosome formation from membrane compartments enriched in phosphatidylinositol 3-phosphate and dynamically connected to the endoplasmic reticulum
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518708/
https://www.ncbi.nlm.nih.gov/pubmed/18725538
http://dx.doi.org/10.1083/jcb.200803137
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