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Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina
PURPOSE: To characterize cyan fluorescent protein (CFP) expression in the retina of the thy1-CFP (B6.Cg-Tg(Thy1-CFP)23Jrs/J) transgenic mouse line. METHODS: CFP expression was characterized using morphometric methods and immunohistochemistry with antibodies to neurofilament light (NF-L), neuronal nu...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Molecular Vision
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519030/ https://www.ncbi.nlm.nih.gov/pubmed/18728756 |
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author | Raymond, Iona D. Vila, Alejandro Huynh, Uyen-Chi N. Brecha, Nicholas C. |
author_facet | Raymond, Iona D. Vila, Alejandro Huynh, Uyen-Chi N. Brecha, Nicholas C. |
author_sort | Raymond, Iona D. |
collection | PubMed |
description | PURPOSE: To characterize cyan fluorescent protein (CFP) expression in the retina of the thy1-CFP (B6.Cg-Tg(Thy1-CFP)23Jrs/J) transgenic mouse line. METHODS: CFP expression was characterized using morphometric methods and immunohistochemistry with antibodies to neurofilament light (NF-L), neuronal nuclei (NeuN), POU-domain protein (Brn3a) and calretinin, which immunolabel ganglion cells, and syntaxin 1 (HPC-1), glutamate decarboxylase 67 (GAD(67)), GABA plasma membrane transporter-1 (GAT-1), and choline acetyltransferase (ChAT), which immunolabel amacrine cells. RESULTS: CFP was extensively expressed in the inner retina, primarily in the inner plexiform layer (IPL), ganglion cell layer (GCL), nerve fiber layer, and optic nerve. CFP fluorescent cell bodies were in all retinal regions and their processes ramified in all laminae of the IPL. Some small, weakly CFP fluorescent somata were in the inner nuclear layer (INL). CFP-containing somata in the GCL ranged from 6 to 20 μm in diameter, and they had a density of 2636±347 cells/mm(2) at 1.5 mm from the optic nerve head. Immunohistochemical studies demonstrated colocalization of CFP with the ganglion cell markers NF-L, NeuN, Brn3a, and calretinin. Immunohistochemistry with antibodies to HPC-1, GAD(67), GAT-1, and ChAT indicated that the small, weakly fluorescent CFP cells in the INL and GCL were cholinergic amacrine cells. CONCLUSIONS: The total number and density of CFP-fluorescent cells in the GCL were within the range of previous estimates of the total number of ganglion cells in the C57BL/6J line. Together these findings suggest that most ganglion cells in the thy1-CFP mouse line 23 express CFP. In conclusion, the thy1-CFP mouse line is highly useful for studies requiring the identification of ganglion cells. |
format | Text |
id | pubmed-2519030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-25190302008-08-26 Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina Raymond, Iona D. Vila, Alejandro Huynh, Uyen-Chi N. Brecha, Nicholas C. Mol Vis Research Article PURPOSE: To characterize cyan fluorescent protein (CFP) expression in the retina of the thy1-CFP (B6.Cg-Tg(Thy1-CFP)23Jrs/J) transgenic mouse line. METHODS: CFP expression was characterized using morphometric methods and immunohistochemistry with antibodies to neurofilament light (NF-L), neuronal nuclei (NeuN), POU-domain protein (Brn3a) and calretinin, which immunolabel ganglion cells, and syntaxin 1 (HPC-1), glutamate decarboxylase 67 (GAD(67)), GABA plasma membrane transporter-1 (GAT-1), and choline acetyltransferase (ChAT), which immunolabel amacrine cells. RESULTS: CFP was extensively expressed in the inner retina, primarily in the inner plexiform layer (IPL), ganglion cell layer (GCL), nerve fiber layer, and optic nerve. CFP fluorescent cell bodies were in all retinal regions and their processes ramified in all laminae of the IPL. Some small, weakly CFP fluorescent somata were in the inner nuclear layer (INL). CFP-containing somata in the GCL ranged from 6 to 20 μm in diameter, and they had a density of 2636±347 cells/mm(2) at 1.5 mm from the optic nerve head. Immunohistochemical studies demonstrated colocalization of CFP with the ganglion cell markers NF-L, NeuN, Brn3a, and calretinin. Immunohistochemistry with antibodies to HPC-1, GAD(67), GAT-1, and ChAT indicated that the small, weakly fluorescent CFP cells in the INL and GCL were cholinergic amacrine cells. CONCLUSIONS: The total number and density of CFP-fluorescent cells in the GCL were within the range of previous estimates of the total number of ganglion cells in the C57BL/6J line. Together these findings suggest that most ganglion cells in the thy1-CFP mouse line 23 express CFP. In conclusion, the thy1-CFP mouse line is highly useful for studies requiring the identification of ganglion cells. Molecular Vision 2008-08-25 /pmc/articles/PMC2519030/ /pubmed/18728756 Text en http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Raymond, Iona D. Vila, Alejandro Huynh, Uyen-Chi N. Brecha, Nicholas C. Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title | Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title_full | Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title_fullStr | Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title_full_unstemmed | Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title_short | Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina |
title_sort | cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-cfp transgenic mouse retina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519030/ https://www.ncbi.nlm.nih.gov/pubmed/18728756 |
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