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Serum MicroRNAs Are Promising Novel Biomarkers

BACKGROUND: Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarke...

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Autores principales: Gilad, Shlomit, Meiri, Eti, Yogev, Yariv, Benjamin, Sima, Lebanony, Danit, Yerushalmi, Noga, Benjamin, Hila, Kushnir, Michal, Cholakh, Hila, Melamed, Nir, Bentwich, Zvi, Hod, Moshe, Goren, Yaron, Chajut, Ayelet
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519789/
https://www.ncbi.nlm.nih.gov/pubmed/18773077
http://dx.doi.org/10.1371/journal.pone.0003148
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author Gilad, Shlomit
Meiri, Eti
Yogev, Yariv
Benjamin, Sima
Lebanony, Danit
Yerushalmi, Noga
Benjamin, Hila
Kushnir, Michal
Cholakh, Hila
Melamed, Nir
Bentwich, Zvi
Hod, Moshe
Goren, Yaron
Chajut, Ayelet
author_facet Gilad, Shlomit
Meiri, Eti
Yogev, Yariv
Benjamin, Sima
Lebanony, Danit
Yerushalmi, Noga
Benjamin, Hila
Kushnir, Michal
Cholakh, Hila
Melamed, Nir
Bentwich, Zvi
Hod, Moshe
Goren, Yaron
Chajut, Ayelet
author_sort Gilad, Shlomit
collection PubMed
description BACKGROUND: Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. METHODS AND RESULTS: After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. CONCLUSIONS AND SIGNIFICANCE: Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers.
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spelling pubmed-25197892008-09-05 Serum MicroRNAs Are Promising Novel Biomarkers Gilad, Shlomit Meiri, Eti Yogev, Yariv Benjamin, Sima Lebanony, Danit Yerushalmi, Noga Benjamin, Hila Kushnir, Michal Cholakh, Hila Melamed, Nir Bentwich, Zvi Hod, Moshe Goren, Yaron Chajut, Ayelet PLoS One Research Article BACKGROUND: Circulating nucleic acids (CNAs) offer unique opportunities for early diagnosis of clinical conditions. Here we show that microRNAs, a family of small non-coding regulatory RNAs involved in human development and pathology, are present in bodily fluids and represent new effective biomarkers. METHODS AND RESULTS: After developing protocols for extracting and quantifying microRNAs in serum and other body fluids, the serum microRNA profiles of several healthy individuals were determined and found to be similar, validating the robustness of our methods. To address the possibility that the abundance of specific microRNAs might change during physiological or pathological conditions, serum microRNA levels in pregnant and non pregnant women were compared. In sera from pregnant women, microRNAs associated with human placenta were significantly elevated and their levels correlated with pregnancy stage. CONCLUSIONS AND SIGNIFICANCE: Considering the central role of microRNAs in development and disease, our results highlight the medically relevant potential of determining microRNA levels in serum and other body fluids. Thus, microRNAs are a new class of CNAs that promise to serve as useful clinical biomarkers. Public Library of Science 2008-09-05 /pmc/articles/PMC2519789/ /pubmed/18773077 http://dx.doi.org/10.1371/journal.pone.0003148 Text en Gilad et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gilad, Shlomit
Meiri, Eti
Yogev, Yariv
Benjamin, Sima
Lebanony, Danit
Yerushalmi, Noga
Benjamin, Hila
Kushnir, Michal
Cholakh, Hila
Melamed, Nir
Bentwich, Zvi
Hod, Moshe
Goren, Yaron
Chajut, Ayelet
Serum MicroRNAs Are Promising Novel Biomarkers
title Serum MicroRNAs Are Promising Novel Biomarkers
title_full Serum MicroRNAs Are Promising Novel Biomarkers
title_fullStr Serum MicroRNAs Are Promising Novel Biomarkers
title_full_unstemmed Serum MicroRNAs Are Promising Novel Biomarkers
title_short Serum MicroRNAs Are Promising Novel Biomarkers
title_sort serum micrornas are promising novel biomarkers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519789/
https://www.ncbi.nlm.nih.gov/pubmed/18773077
http://dx.doi.org/10.1371/journal.pone.0003148
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