Improved plant transformation vectors for fluorescent protein tagging

Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a serie...

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Detalles Bibliográficos
Autores principales: Zhong, Silin, Lin, Zhefeng, Fray, Rupert G., Grierson, Don
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2008
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522295/
https://www.ncbi.nlm.nih.gov/pubmed/18594998
http://dx.doi.org/10.1007/s11248-008-9199-y
Descripción
Sumario:Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a series of 23 novel GATEWAY-compatible vectors based on pGreenII and pDH51 backbones with the latest fluorescent protein tags (Cerulean, EGFP and Venus) and the choice of three in planta selection markers. These vectors can be obtained from the Nottingham Arabidopsis Stock Centre (N9819-N9846) and should be a powerful tool box for transgenic research in plants.

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