Improved plant transformation vectors for fluorescent protein tagging

Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a serie...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhong, Silin, Lin, Zhefeng, Fray, Rupert G., Grierson, Don
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2008
Materias:
Brief Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522295/
https://www.ncbi.nlm.nih.gov/pubmed/18594998
http://dx.doi.org/10.1007/s11248-008-9199-y
_version_ 1782158648284282880
author Zhong, Silin
Lin, Zhefeng
Fray, Rupert G.
Grierson, Don
author_facet Zhong, Silin
Lin, Zhefeng
Fray, Rupert G.
Grierson, Don
author_sort Zhong, Silin
collection PubMed
description Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a series of 23 novel GATEWAY-compatible vectors based on pGreenII and pDH51 backbones with the latest fluorescent protein tags (Cerulean, EGFP and Venus) and the choice of three in planta selection markers. These vectors can be obtained from the Nottingham Arabidopsis Stock Centre (N9819-N9846) and should be a powerful tool box for transgenic research in plants.
format Text
id pubmed-2522295
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-25222952008-08-27 Improved plant transformation vectors for fluorescent protein tagging Zhong, Silin Lin, Zhefeng Fray, Rupert G. Grierson, Don Transgenic Res Brief Communication Fluorescent protein labelling technologies enable dynamic protein actions to be imaged in living cells and can also be used in conjunction with other methods such as Forster resonance energy transfer and biomolecular fluorescence complementation. In this report, we describe the generation of a series of 23 novel GATEWAY-compatible vectors based on pGreenII and pDH51 backbones with the latest fluorescent protein tags (Cerulean, EGFP and Venus) and the choice of three in planta selection markers. These vectors can be obtained from the Nottingham Arabidopsis Stock Centre (N9819-N9846) and should be a powerful tool box for transgenic research in plants. Springer Netherlands 2008-07-02 2008 /pmc/articles/PMC2522295/ /pubmed/18594998 http://dx.doi.org/10.1007/s11248-008-9199-y Text en © The Author(s) 2008 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Brief Communication
Zhong, Silin
Lin, Zhefeng
Fray, Rupert G.
Grierson, Don
Improved plant transformation vectors for fluorescent protein tagging
title Improved plant transformation vectors for fluorescent protein tagging
title_full Improved plant transformation vectors for fluorescent protein tagging
title_fullStr Improved plant transformation vectors for fluorescent protein tagging
title_full_unstemmed Improved plant transformation vectors for fluorescent protein tagging
title_short Improved plant transformation vectors for fluorescent protein tagging
title_sort improved plant transformation vectors for fluorescent protein tagging
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522295/
https://www.ncbi.nlm.nih.gov/pubmed/18594998
http://dx.doi.org/10.1007/s11248-008-9199-y
work_keys_str_mv AT zhongsilin improvedplanttransformationvectorsforfluorescentproteintagging
AT linzhefeng improvedplanttransformationvectorsforfluorescentproteintagging
AT frayrupertg improvedplanttransformationvectorsforfluorescentproteintagging
AT griersondon improvedplanttransformationvectorsforfluorescentproteintagging

Ejemplares similares