Cargando…

Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration

Dendritic cells (DC) are professional antigen-presenting cells of the immune system that play a key role in regulating T cell-based immunity. In vivo, the capacity of DC to activate T cells depends on their ability to migrate to the T cell areas of lymph nodes as well as on their maturation state. D...

Descripción completa

Detalles Bibliográficos
Autores principales: Boullart, A. C. Inge, Aarntzen, Erik H. J. G., Verdijk, Pauline, Jacobs, Joannes F. M., Schuurhuis, Danita H., Benitez-Ribas, Daniel, Schreibelt, Gerty, van de Rakt, Mandy W. M. M., Scharenborg, Nicole M., de Boer, Annemiek, Kramer, Matthijs, Figdor, Carl G., Punt, Cornelis J. A., Adema, Gosse J., de Vries, I. Jolanda M.
Formato: Texto
Lenguaje:English
Publicado: Springer-Verlag 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522299/
https://www.ncbi.nlm.nih.gov/pubmed/18322684
http://dx.doi.org/10.1007/s00262-008-0489-2
_version_ 1782158649194446848
author Boullart, A. C. Inge
Aarntzen, Erik H. J. G.
Verdijk, Pauline
Jacobs, Joannes F. M.
Schuurhuis, Danita H.
Benitez-Ribas, Daniel
Schreibelt, Gerty
van de Rakt, Mandy W. M. M.
Scharenborg, Nicole M.
de Boer, Annemiek
Kramer, Matthijs
Figdor, Carl G.
Punt, Cornelis J. A.
Adema, Gosse J.
de Vries, I. Jolanda M.
author_facet Boullart, A. C. Inge
Aarntzen, Erik H. J. G.
Verdijk, Pauline
Jacobs, Joannes F. M.
Schuurhuis, Danita H.
Benitez-Ribas, Daniel
Schreibelt, Gerty
van de Rakt, Mandy W. M. M.
Scharenborg, Nicole M.
de Boer, Annemiek
Kramer, Matthijs
Figdor, Carl G.
Punt, Cornelis J. A.
Adema, Gosse J.
de Vries, I. Jolanda M.
author_sort Boullart, A. C. Inge
collection PubMed
description Dendritic cells (DC) are professional antigen-presenting cells of the immune system that play a key role in regulating T cell-based immunity. In vivo, the capacity of DC to activate T cells depends on their ability to migrate to the T cell areas of lymph nodes as well as on their maturation state. Depending on their cytokine-secreting profile, DC are able to skew the immune response in a specific direction. In particular, IL-12p70 producing DC drive T cells towards a T helper 1 type response. A serious disadvantage of current clinical grade ex vivo generated monocyte-derived DC is the poor IL-12p70 production. We have investigated the effects of Toll-like receptor (TLR)-mediated maturation on ex vivo generated human monocyte-derived DC. We demonstrate that in contrast to cytokine-matured DC, DC matured with poly(I:C) (TLR3 ligand) and/or R848 (TLR7/8 ligand) are able to produce vast amounts of IL-12p70, but exhibit a reduced migratory capacity. The addition of prostaglandin E(2) (PGE(2)) improved the migratory capacity of TLR-ligand matured DC while maintaining their IL-12p70 production upon T cell encounter. We propose a novel clinical grade maturation protocol in which TLR ligands poly(I:C) and R848 are combined with PGE(2) to generate DC with both high migratory capacity and IL-12p70 production upon T cell encounter. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00262-008-0489-2) contains supplementary material, which is available to authorized users.
format Text
id pubmed-2522299
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Springer-Verlag
record_format MEDLINE/PubMed
spelling pubmed-25222992008-08-27 Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration Boullart, A. C. Inge Aarntzen, Erik H. J. G. Verdijk, Pauline Jacobs, Joannes F. M. Schuurhuis, Danita H. Benitez-Ribas, Daniel Schreibelt, Gerty van de Rakt, Mandy W. M. M. Scharenborg, Nicole M. de Boer, Annemiek Kramer, Matthijs Figdor, Carl G. Punt, Cornelis J. A. Adema, Gosse J. de Vries, I. Jolanda M. Cancer Immunol Immunother Original Article Dendritic cells (DC) are professional antigen-presenting cells of the immune system that play a key role in regulating T cell-based immunity. In vivo, the capacity of DC to activate T cells depends on their ability to migrate to the T cell areas of lymph nodes as well as on their maturation state. Depending on their cytokine-secreting profile, DC are able to skew the immune response in a specific direction. In particular, IL-12p70 producing DC drive T cells towards a T helper 1 type response. A serious disadvantage of current clinical grade ex vivo generated monocyte-derived DC is the poor IL-12p70 production. We have investigated the effects of Toll-like receptor (TLR)-mediated maturation on ex vivo generated human monocyte-derived DC. We demonstrate that in contrast to cytokine-matured DC, DC matured with poly(I:C) (TLR3 ligand) and/or R848 (TLR7/8 ligand) are able to produce vast amounts of IL-12p70, but exhibit a reduced migratory capacity. The addition of prostaglandin E(2) (PGE(2)) improved the migratory capacity of TLR-ligand matured DC while maintaining their IL-12p70 production upon T cell encounter. We propose a novel clinical grade maturation protocol in which TLR ligands poly(I:C) and R848 are combined with PGE(2) to generate DC with both high migratory capacity and IL-12p70 production upon T cell encounter. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00262-008-0489-2) contains supplementary material, which is available to authorized users. Springer-Verlag 2008-03-06 2008 /pmc/articles/PMC2522299/ /pubmed/18322684 http://dx.doi.org/10.1007/s00262-008-0489-2 Text en © The Author(s) 2008 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Boullart, A. C. Inge
Aarntzen, Erik H. J. G.
Verdijk, Pauline
Jacobs, Joannes F. M.
Schuurhuis, Danita H.
Benitez-Ribas, Daniel
Schreibelt, Gerty
van de Rakt, Mandy W. M. M.
Scharenborg, Nicole M.
de Boer, Annemiek
Kramer, Matthijs
Figdor, Carl G.
Punt, Cornelis J. A.
Adema, Gosse J.
de Vries, I. Jolanda M.
Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title_full Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title_fullStr Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title_full_unstemmed Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title_short Maturation of monocyte-derived dendritic cells with Toll-like receptor 3 and 7/8 ligands combined with prostaglandin E(2) results in high interleukin-12 production and cell migration
title_sort maturation of monocyte-derived dendritic cells with toll-like receptor 3 and 7/8 ligands combined with prostaglandin e(2) results in high interleukin-12 production and cell migration
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522299/
https://www.ncbi.nlm.nih.gov/pubmed/18322684
http://dx.doi.org/10.1007/s00262-008-0489-2
work_keys_str_mv AT boullartacinge maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT aarntzenerikhjg maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT verdijkpauline maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT jacobsjoannesfm maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT schuurhuisdanitah maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT benitezribasdaniel maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT schreibeltgerty maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT vanderaktmandywmm maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT scharenborgnicolem maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT deboerannemiek maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT kramermatthijs maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT figdorcarlg maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT puntcornelisja maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT ademagossej maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration
AT devriesijolandam maturationofmonocytederiveddendriticcellswithtolllikereceptor3and78ligandscombinedwithprostaglandine2resultsinhighinterleukin12productionandcellmigration