Cargando…

Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease

Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer’s disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into...

Descripción completa

Detalles Bibliográficos
Autores principales: Youm, Jung Won, Jeon, Jae Heung, Kim, Hee, Kim, Young Ho, Ko, Kisung, Joung, Hyouk, Kim, HyunSoon
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522325/
https://www.ncbi.nlm.nih.gov/pubmed/18604480
http://dx.doi.org/10.1007/s10529-008-9759-5
_version_ 1782158650596392960
author Youm, Jung Won
Jeon, Jae Heung
Kim, Hee
Kim, Young Ho
Ko, Kisung
Joung, Hyouk
Kim, HyunSoon
author_facet Youm, Jung Won
Jeon, Jae Heung
Kim, Hee
Kim, Young Ho
Ko, Kisung
Joung, Hyouk
Kim, HyunSoon
author_sort Youm, Jung Won
collection PubMed
description Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer’s disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen.
format Text
id pubmed-2522325
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-25223252008-08-27 Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease Youm, Jung Won Jeon, Jae Heung Kim, Hee Kim, Young Ho Ko, Kisung Joung, Hyouk Kim, HyunSoon Biotechnol Lett Original Research Paper Human β-amyloid (Aβ) is believed to be one of the main components of Alzheimer’s disease, so reduction of Aβ is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Aβ with tandem repeats. Integration of the human Aβ gene into the tomato genome and its transcription were detected by PCR and Northern blot, respectively. Expression of the Aβ protein was confirmed by western blot and ELISA, and then the transgenic tomato line expressing the highest protein level was selected for vaccination. Mice immunized orally with total soluble extracts from the transgenic tomato plants elicited an immune response after receiving a booster. The results indicate that tomato plants may provide a useful system for the production of human Aβ antigen. Springer Netherlands 2008-07-05 2008-10 /pmc/articles/PMC2522325/ /pubmed/18604480 http://dx.doi.org/10.1007/s10529-008-9759-5 Text en © Springer Science+Business Media B.V. 2008
spellingShingle Original Research Paper
Youm, Jung Won
Jeon, Jae Heung
Kim, Hee
Kim, Young Ho
Ko, Kisung
Joung, Hyouk
Kim, HyunSoon
Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title_full Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title_fullStr Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title_full_unstemmed Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title_short Transgenic tomatoes expressing human beta-amyloid for use as a vaccine against Alzheimer’s disease
title_sort transgenic tomatoes expressing human beta-amyloid for use as a vaccine against alzheimer’s disease
topic Original Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2522325/
https://www.ncbi.nlm.nih.gov/pubmed/18604480
http://dx.doi.org/10.1007/s10529-008-9759-5
work_keys_str_mv AT youmjungwon transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT jeonjaeheung transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT kimhee transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT kimyoungho transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT kokisung transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT jounghyouk transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease
AT kimhyunsoon transgenictomatoesexpressinghumanbetaamyloidforuseasavaccineagainstalzheimersdisease