Cargando…
Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat)
Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The ob...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2525491/ https://www.ncbi.nlm.nih.gov/pubmed/18728729 http://dx.doi.org/10.3390/md20080014 |
_version_ | 1782158658364243968 |
---|---|
author | Walsh, Catherine J. Leggett, Stephanie R. Strohbehn, Kathryn Pierce, Richard H. Sleasman, John W. |
author_facet | Walsh, Catherine J. Leggett, Stephanie R. Strohbehn, Kathryn Pierce, Richard H. Sleasman, John W. |
author_sort | Walsh, Catherine J. |
collection | PubMed |
description | Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The objective of this research was to investigate potential immunotoxic effects of brevetoxins using a leukemic T cell line (Jurkat) as an in vitro model system. Viability, cell proliferation, and apoptosis assays were conducted using brevetoxin congeners PbTx-2, PbTx-3, and PbTx-6. The effects of in vitro brevetoxin exposure on cell viability and cellular metabolism or proliferation were determined using trypan blue and MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan), respectively. Using MTT, cellular metabolic activity was decreased in Jurkat cells exposed to 5 – 10 μg/ml PbTx-2 or PbTx-6. After 3 h, no significant effects on cell viability were observed with any toxin congener in concentrations up to 10 μg/ml. Viability decreased dramatically after 24 h in cells treated with PbTx-2 or -6. Apoptosis, as measured by caspase-3 activity, was significantly increased in cells exposed to PbTx-2 or PbTx-6. In summary, brevetoxin congeners varied in effects on Jurkat cells, with PbTx-2 and PbTx-6 eliciting greater cellular effects compared to PbTx-3. |
format | Text |
id | pubmed-2525491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International |
record_format | MEDLINE/PubMed |
spelling | pubmed-25254912008-08-26 Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) Walsh, Catherine J. Leggett, Stephanie R. Strohbehn, Kathryn Pierce, Richard H. Sleasman, John W. Mar Drugs Article Harmful algal blooms (HABs) of the toxic dinoflagellate, Karenia brevis, produce red tide toxins, or brevetoxins. Significant health effects associated with red tide toxin exposure have been reported in sea life and in humans, with brevetoxins documented within immune cells from many species. The objective of this research was to investigate potential immunotoxic effects of brevetoxins using a leukemic T cell line (Jurkat) as an in vitro model system. Viability, cell proliferation, and apoptosis assays were conducted using brevetoxin congeners PbTx-2, PbTx-3, and PbTx-6. The effects of in vitro brevetoxin exposure on cell viability and cellular metabolism or proliferation were determined using trypan blue and MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan), respectively. Using MTT, cellular metabolic activity was decreased in Jurkat cells exposed to 5 – 10 μg/ml PbTx-2 or PbTx-6. After 3 h, no significant effects on cell viability were observed with any toxin congener in concentrations up to 10 μg/ml. Viability decreased dramatically after 24 h in cells treated with PbTx-2 or -6. Apoptosis, as measured by caspase-3 activity, was significantly increased in cells exposed to PbTx-2 or PbTx-6. In summary, brevetoxin congeners varied in effects on Jurkat cells, with PbTx-2 and PbTx-6 eliciting greater cellular effects compared to PbTx-3. Molecular Diversity Preservation International 2008-06-10 /pmc/articles/PMC2525491/ /pubmed/18728729 http://dx.doi.org/10.3390/md20080014 Text en © 2008 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland |
spellingShingle | Article Walsh, Catherine J. Leggett, Stephanie R. Strohbehn, Kathryn Pierce, Richard H. Sleasman, John W. Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title | Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title_full | Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title_fullStr | Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title_full_unstemmed | Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title_short | Effects of in vitro Brevetoxin Exposure on Apoptosis and Cellular Metabolism in a Leukemic T Cell Line (Jurkat) |
title_sort | effects of in vitro brevetoxin exposure on apoptosis and cellular metabolism in a leukemic t cell line (jurkat) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2525491/ https://www.ncbi.nlm.nih.gov/pubmed/18728729 http://dx.doi.org/10.3390/md20080014 |
work_keys_str_mv | AT walshcatherinej effectsofinvitrobrevetoxinexposureonapoptosisandcellularmetabolisminaleukemictcelllinejurkat AT leggettstephanier effectsofinvitrobrevetoxinexposureonapoptosisandcellularmetabolisminaleukemictcelllinejurkat AT strohbehnkathryn effectsofinvitrobrevetoxinexposureonapoptosisandcellularmetabolisminaleukemictcelllinejurkat AT piercerichardh effectsofinvitrobrevetoxinexposureonapoptosisandcellularmetabolisminaleukemictcelllinejurkat AT sleasmanjohnw effectsofinvitrobrevetoxinexposureonapoptosisandcellularmetabolisminaleukemictcelllinejurkat |