Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo

Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500–750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons c...

Descripción completa

Detalles Bibliográficos
Autores principales: Moreaux, Laurent, Laurent, Gilles
Formato: Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2007
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526277/
https://www.ncbi.nlm.nih.gov/pubmed/18946544
http://dx.doi.org/10.3389/neuro.04.002.2007
_version_ 1782158735885467648
author Moreaux, Laurent
Laurent, Gilles
author_facet Moreaux, Laurent
Laurent, Gilles
author_sort Moreaux, Laurent
collection PubMed
description Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500–750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated in vivo with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80–90% accuracy and 50 ms temporal resolution.
format Text
id pubmed-2526277
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Frontiers Research Foundation
record_format MEDLINE/PubMed
spelling pubmed-25262772008-10-22 Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo Moreaux, Laurent Laurent, Gilles Front Neural Circuits Neuroscience Combining intracellular electrophysiology and multi-photon calcium imaging in vivo, we studied the relationship between calcium signals (sampled at 500–750 Hz) and spike output in principal neurons in the locust antennal lobe. Our goal was to determine whether the firing rate of individual neurons can be estimated in vivo with calcium imaging and, if so, to measure directly the accuracy and resolution of our estimates. Using the calcium indicator Oregon Green BAPTA-1, we describe a simple method to reconstruct firing rates from dendritic calcium signals with 80–90% accuracy and 50 ms temporal resolution. Frontiers Research Foundation 2007-11-02 /pmc/articles/PMC2526277/ /pubmed/18946544 http://dx.doi.org/10.3389/neuro.04.002.2007 Text en Copyright: © 2007 Moreaux, Laurent. http://www.frontiersin.org/licenseagreement This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
spellingShingle Neuroscience
Moreaux, Laurent
Laurent, Gilles
Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title_full Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title_fullStr Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title_full_unstemmed Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title_short Estimating Firing Rates from Calcium Signals in Locust Projection Neurons in Vivo
title_sort estimating firing rates from calcium signals in locust projection neurons in vivo
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526277/
https://www.ncbi.nlm.nih.gov/pubmed/18946544
http://dx.doi.org/10.3389/neuro.04.002.2007
work_keys_str_mv AT moreauxlaurent estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsinvivo
AT laurentgilles estimatingfiringratesfromcalciumsignalsinlocustprojectionneuronsinvivo

Ejemplares similares