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Quantitative measurements of C-reactive protein using silicon nanowire arrays

A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interactions such as protein disease markers, viruses, and DNA hybridization. Furthermore, because the silicon nanowire can display results in real-time, it...

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Detalles Bibliográficos
Autores principales: Lee, Min-Ho, Lee, Kuk-Nyung, Jung, Suk-Won, Kim, Won-Hyo, Shin, Kyu-Sik, Seong, Woo-Kyeong
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526356/
https://www.ncbi.nlm.nih.gov/pubmed/18488422
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author Lee, Min-Ho
Lee, Kuk-Nyung
Jung, Suk-Won
Kim, Won-Hyo
Shin, Kyu-Sik
Seong, Woo-Kyeong
author_facet Lee, Min-Ho
Lee, Kuk-Nyung
Jung, Suk-Won
Kim, Won-Hyo
Shin, Kyu-Sik
Seong, Woo-Kyeong
author_sort Lee, Min-Ho
collection PubMed
description A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interactions such as protein disease markers, viruses, and DNA hybridization. Furthermore, because the silicon nanowire can display results in real-time, it may possess superior characteristics for biosensing than those demonstrated in previously studied methods. However, despite its promising potential and advantages, certain process-related limitations of the device, due to its size and material characteristics, need to be addressed. In this article, we suggest possible solutions. We fabricated silicon nanowire using a top-down and low cost micromachining method, and evaluate the sensing of molecules after transfer and surface modifications. Our newly designed method can be used to attach highly ordered nanowires to various substrates, to form a nanowire array device, which needs to follow a series of repetitive steps in conventional fabrication technology based on a vapor-liquid-solid (VLS) method. For evaluation, we demonstrated that our newly fabricated silicon nanowire arrays could detect pH changes as well as streptavidin-biotin binding events. As well as the initial proof-of-principle studies, C-reactive protein binding was measured: electrical signals were changed in a linear fashion with the concentration (1 fM to 1 nM) in PBS containing 1.37 mM of salts. Finally, to address the effects of Debye length, silicon nanowires coupled with antigen proteins underwent electrical signal changes as the salt concentration changed.
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spelling pubmed-25263562008-09-04 Quantitative measurements of C-reactive protein using silicon nanowire arrays Lee, Min-Ho Lee, Kuk-Nyung Jung, Suk-Won Kim, Won-Hyo Shin, Kyu-Sik Seong, Woo-Kyeong Int J Nanomedicine Case Report A silicon nanowire-based sensor for biological application showed highly desirable electrical responses to either pH changes or receptor-ligand interactions such as protein disease markers, viruses, and DNA hybridization. Furthermore, because the silicon nanowire can display results in real-time, it may possess superior characteristics for biosensing than those demonstrated in previously studied methods. However, despite its promising potential and advantages, certain process-related limitations of the device, due to its size and material characteristics, need to be addressed. In this article, we suggest possible solutions. We fabricated silicon nanowire using a top-down and low cost micromachining method, and evaluate the sensing of molecules after transfer and surface modifications. Our newly designed method can be used to attach highly ordered nanowires to various substrates, to form a nanowire array device, which needs to follow a series of repetitive steps in conventional fabrication technology based on a vapor-liquid-solid (VLS) method. For evaluation, we demonstrated that our newly fabricated silicon nanowire arrays could detect pH changes as well as streptavidin-biotin binding events. As well as the initial proof-of-principle studies, C-reactive protein binding was measured: electrical signals were changed in a linear fashion with the concentration (1 fM to 1 nM) in PBS containing 1.37 mM of salts. Finally, to address the effects of Debye length, silicon nanowires coupled with antigen proteins underwent electrical signal changes as the salt concentration changed. Dove Medical Press 2008-03 2008-03 /pmc/articles/PMC2526356/ /pubmed/18488422 Text en © 2008 Lee et al, publisher and licensee Dove Medical Press Ltd.
spellingShingle Case Report
Lee, Min-Ho
Lee, Kuk-Nyung
Jung, Suk-Won
Kim, Won-Hyo
Shin, Kyu-Sik
Seong, Woo-Kyeong
Quantitative measurements of C-reactive protein using silicon nanowire arrays
title Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_full Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_fullStr Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_full_unstemmed Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_short Quantitative measurements of C-reactive protein using silicon nanowire arrays
title_sort quantitative measurements of c-reactive protein using silicon nanowire arrays
topic Case Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526356/
https://www.ncbi.nlm.nih.gov/pubmed/18488422
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