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Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles

Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions...

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Detalles Bibliográficos
Autores principales: Vamanu, Carmen Irina, Høl, Paul Johan, Allouni, Zouhir Ekeland, Elsayed, Said, Gjerdet, Nils Roar
Formato: Texto
Lenguaje:English
Publicado: Dove Medical Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526361/
https://www.ncbi.nlm.nih.gov/pubmed/18488417
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author Vamanu, Carmen Irina
Høl, Paul Johan
Allouni, Zouhir Ekeland
Elsayed, Said
Gjerdet, Nils Roar
author_facet Vamanu, Carmen Irina
Høl, Paul Johan
Allouni, Zouhir Ekeland
Elsayed, Said
Gjerdet, Nils Roar
author_sort Vamanu, Carmen Irina
collection PubMed
description Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO(2) nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO(2) nanoparticles in cell culture medium. It is shown that nano TiO(2)-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests.
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spelling pubmed-25263612008-09-04 Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles Vamanu, Carmen Irina Høl, Paul Johan Allouni, Zouhir Ekeland Elsayed, Said Gjerdet, Nils Roar Int J Nanomedicine Original Research Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO(2) nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO(2) nanoparticles in cell culture medium. It is shown that nano TiO(2)-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests. Dove Medical Press 2008-03 2008-03 /pmc/articles/PMC2526361/ /pubmed/18488417 Text en © 2008 Dove Medical Press Limited. All rights reserved
spellingShingle Original Research
Vamanu, Carmen Irina
Høl, Paul Johan
Allouni, Zouhir Ekeland
Elsayed, Said
Gjerdet, Nils Roar
Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_full Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_fullStr Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_full_unstemmed Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_short Formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
title_sort formation of potential titanium antigens based on protein binding to titanium dioxide nanoparticles
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526361/
https://www.ncbi.nlm.nih.gov/pubmed/18488417
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