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Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model

BACKGROUND: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving gr...

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Autores principales: Leichsenring, Anna, Bäcker, Ingo, Wendt, Wiebke, Andriske, Michael, Schmitz, Beate, Stichel, Christine C, Lübbert, Hermann
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2527007/
https://www.ncbi.nlm.nih.gov/pubmed/18700000
http://dx.doi.org/10.1186/1471-2202-9-80
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author Leichsenring, Anna
Bäcker, Ingo
Wendt, Wiebke
Andriske, Michael
Schmitz, Beate
Stichel, Christine C
Lübbert, Hermann
author_facet Leichsenring, Anna
Bäcker, Ingo
Wendt, Wiebke
Andriske, Michael
Schmitz, Beate
Stichel, Christine C
Lübbert, Hermann
author_sort Leichsenring, Anna
collection PubMed
description BACKGROUND: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5(th )lumbar spinal nerve transection model (L5T). RESULTS: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different. CONCLUSION: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance.
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spelling pubmed-25270072008-08-29 Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model Leichsenring, Anna Bäcker, Ingo Wendt, Wiebke Andriske, Michael Schmitz, Beate Stichel, Christine C Lübbert, Hermann BMC Neurosci Research Article BACKGROUND: Ample evidence suggests a substantial contribution of cellular and molecular changes in the spinal cord to the induction and persistence of chronic neuropathic pain conditions. While for a long time, proteases were mainly considered as protein degrading enzymes, they are now receiving growing interest as signalling molecules in the pain pathology. In the present study we focused on two cathepsins, CATS and CATX, and studied their spatiotemporal expression and activity during the development and progression of neuropathic pain in the CNS of the rat 5(th )lumbar spinal nerve transection model (L5T). RESULTS: Immediately after the lesion, both cathepsins, CATS and CATX, were upregulated in the spinal cord. Moreover, we succeeded in measuring the activity of CATX, which was substantially increased after L5T. The differential expression of these proteins exhibited the same spatial distribution and temporal progression in the spinal cord, progressing up to the medulla oblongata in the late phase of chronic pain. The cellular distribution of CATS and CATX was, however, considerably different. CONCLUSION: The cellular distribution and the spatio-temporal development of the altered expression of CATS and CATX suggest that these proteins are important players in the spinal mechanisms involved in chronic pain induction and maintenance. BioMed Central 2008-08-12 /pmc/articles/PMC2527007/ /pubmed/18700000 http://dx.doi.org/10.1186/1471-2202-9-80 Text en Copyright © 2008 Leichsenring et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Leichsenring, Anna
Bäcker, Ingo
Wendt, Wiebke
Andriske, Michael
Schmitz, Beate
Stichel, Christine C
Lübbert, Hermann
Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title_full Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title_fullStr Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title_full_unstemmed Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title_short Differential expression of Cathepsin S and X in the spinal cord of a rat neuropathic pain model
title_sort differential expression of cathepsin s and x in the spinal cord of a rat neuropathic pain model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2527007/
https://www.ncbi.nlm.nih.gov/pubmed/18700000
http://dx.doi.org/10.1186/1471-2202-9-80
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