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Plasmid selection in Escherichia coli using an endogenous essential gene marker
BACKGROUND: Antibiotic resistance genes are widely used for selection of recombinant bacteria, but their use risks contributing to the spread of antibiotic resistance. In particular, the practice is inappropriate for some intrinsically resistant bacteria and in vaccine production, and costly for ind...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2527308/ https://www.ncbi.nlm.nih.gov/pubmed/18694482 http://dx.doi.org/10.1186/1472-6750-8-61 |