Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfection protocol that yields titers exceeding 1...

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Detalles Bibliográficos
Autores principales: Backliwal, Gaurav, Hildinger, Markus, Chenuet, Sebastien, Wulhfard, Sarah, De Jesus, Maria, Wurm, Florian M.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528171/
https://www.ncbi.nlm.nih.gov/pubmed/18617574
http://dx.doi.org/10.1093/nar/gkn423

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528171/
https://www.ncbi.nlm.nih.gov/pubmed/18617574
http://dx.doi.org/10.1093/nar/gkn423

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