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The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression

Inner nuclear membrane proteins containing a LEM (LAP2, emerin, and MAN1) domain participate in different processes, including chromatin organization, gene expression, and nuclear envelope biogenesis. In this study, we identify a robust genetic interaction between transcription export (TREX) factors...

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Autores principales: Grund, Stefanie E., Fischer, Tamás, Cabal, Ghislain G., Antúnez, Oreto, Pérez-Ortín, José E., Hurt, Ed
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528585/
https://www.ncbi.nlm.nih.gov/pubmed/18762579
http://dx.doi.org/10.1083/jcb.200803098
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author Grund, Stefanie E.
Fischer, Tamás
Cabal, Ghislain G.
Antúnez, Oreto
Pérez-Ortín, José E.
Hurt, Ed
author_facet Grund, Stefanie E.
Fischer, Tamás
Cabal, Ghislain G.
Antúnez, Oreto
Pérez-Ortín, José E.
Hurt, Ed
author_sort Grund, Stefanie E.
collection PubMed
description Inner nuclear membrane proteins containing a LEM (LAP2, emerin, and MAN1) domain participate in different processes, including chromatin organization, gene expression, and nuclear envelope biogenesis. In this study, we identify a robust genetic interaction between transcription export (TREX) factors and yeast Src1, an integral inner nuclear membrane protein that is homologous to vertebrate LEM2. DNA macroarray analysis revealed that the expression of the phosphate-regulated genes PHO11, PHO12, and PHO84 is up-regulated in src1Δ cells. Notably, these PHO genes are located in subtelomeric regions of chromatin and exhibit a perinuclear location in vivo. Src1 spans the nuclear membrane twice and exposes its N and C domains with putative DNA-binding motifs to the nucleoplasm. Genome-wide chromatin immunoprecipitation–on-chip analyses indicated that Src1 is highly enriched at telomeres and subtelomeric regions of the yeast chromosomes. Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes.
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spelling pubmed-25285852009-03-08 The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression Grund, Stefanie E. Fischer, Tamás Cabal, Ghislain G. Antúnez, Oreto Pérez-Ortín, José E. Hurt, Ed J Cell Biol Research Articles Inner nuclear membrane proteins containing a LEM (LAP2, emerin, and MAN1) domain participate in different processes, including chromatin organization, gene expression, and nuclear envelope biogenesis. In this study, we identify a robust genetic interaction between transcription export (TREX) factors and yeast Src1, an integral inner nuclear membrane protein that is homologous to vertebrate LEM2. DNA macroarray analysis revealed that the expression of the phosphate-regulated genes PHO11, PHO12, and PHO84 is up-regulated in src1Δ cells. Notably, these PHO genes are located in subtelomeric regions of chromatin and exhibit a perinuclear location in vivo. Src1 spans the nuclear membrane twice and exposes its N and C domains with putative DNA-binding motifs to the nucleoplasm. Genome-wide chromatin immunoprecipitation–on-chip analyses indicated that Src1 is highly enriched at telomeres and subtelomeric regions of the yeast chromosomes. Our data show that the inner nuclear membrane protein Src1 functions at the interface between subtelomeric gene expression and TREX-dependent messenger RNA export through the nuclear pore complexes. The Rockefeller University Press 2008-09-08 /pmc/articles/PMC2528585/ /pubmed/18762579 http://dx.doi.org/10.1083/jcb.200803098 Text en © 2008 Grund et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Grund, Stefanie E.
Fischer, Tamás
Cabal, Ghislain G.
Antúnez, Oreto
Pérez-Ortín, José E.
Hurt, Ed
The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title_full The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title_fullStr The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title_full_unstemmed The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title_short The inner nuclear membrane protein Src1 associates with subtelomeric genes and alters their regulated gene expression
title_sort inner nuclear membrane protein src1 associates with subtelomeric genes and alters their regulated gene expression
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528585/
https://www.ncbi.nlm.nih.gov/pubmed/18762579
http://dx.doi.org/10.1083/jcb.200803098
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