Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

BACKGROUND: The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory controls. The construction of plasmid-less marker-less str...

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Detalles Bibliográficos
Autores principales: Minaeva, Natalia I, Gak, Evgeny R, Zimenkov, Danila V, Skorokhodova, Aleksandra Yu, Biryukova, Irina V, Mashko, Sergey V
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532685/
https://www.ncbi.nlm.nih.gov/pubmed/18699991
http://dx.doi.org/10.1186/1472-6750-8-63

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