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Ultrasound-guided in utero injections allow studies of the development and function of the eye
Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We det...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Wiley-Liss, Inc.
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532702/ https://www.ncbi.nlm.nih.gov/pubmed/18351670 http://dx.doi.org/10.1002/dvdy.21500 |
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author | Punzo, Claudio Cepko, Constance L. |
author_facet | Punzo, Claudio Cepko, Constance L. |
author_sort | Punzo, Claudio |
collection | PubMed |
description | Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We determined the window of time, as well as compared several strains of mice, that yield a high degree of survival and successful gene transfer. Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types. In addition, a retroviral vector was engineered to give cone photoreceptor-enriched expression, and a retroviral vector was demonstrated to provide RNAi-mediated loss-of-function. These methods enable access to early ocular structures and provide a more rapid method of assessment of gene and promoter function than possible using genetically engineered mice. |
format | Text |
id | pubmed-2532702 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Wiley-Liss, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-25327022009-04-17 Ultrasound-guided in utero injections allow studies of the development and function of the eye Punzo, Claudio Cepko, Constance L. Dev Dyn Techniques Ultrasound-guided in utero injections into the brain of murine embryos has been shown to facilitate gene delivery. We investigated whether these methods would allow gene transfer into ocular structures. Gene transfer using retroviral vectors or electroporation was found to be quite effective. We determined the window of time, as well as compared several strains of mice, that yield a high degree of survival and successful gene transfer. Several retroviral constructs were tested for expression and coexpresssion of two genes in retinal cell types. In addition, a retroviral vector was engineered to give cone photoreceptor-enriched expression, and a retroviral vector was demonstrated to provide RNAi-mediated loss-of-function. These methods enable access to early ocular structures and provide a more rapid method of assessment of gene and promoter function than possible using genetically engineered mice. Wiley-Liss, Inc. 2008-04 2008-03-20 /pmc/articles/PMC2532702/ /pubmed/18351670 http://dx.doi.org/10.1002/dvdy.21500 Text en Copyright © 2008 Wiley-Liss, Inc. |
spellingShingle | Techniques Punzo, Claudio Cepko, Constance L. Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title | Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title_full | Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title_fullStr | Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title_full_unstemmed | Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title_short | Ultrasound-guided in utero injections allow studies of the development and function of the eye |
title_sort | ultrasound-guided in utero injections allow studies of the development and function of the eye |
topic | Techniques |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2532702/ https://www.ncbi.nlm.nih.gov/pubmed/18351670 http://dx.doi.org/10.1002/dvdy.21500 |
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