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A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
BACKGROUND: Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (Ct) method is the present "gold standard", it is far from being a standard...
Autores principales: | Guescini, Michele, Sisti, Davide, Rocchi, Marco BL, Stocchi, Laura, Stocchi, Vilberto |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533027/ https://www.ncbi.nlm.nih.gov/pubmed/18667053 http://dx.doi.org/10.1186/1471-2105-9-326 |
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