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Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis

Chromosome movements are a general feature of mid-prophase of meiosis. In budding yeast, meiotic chromosomes exhibit dynamic movements, led by nuclear envelope (NE)-associated telomeres, throughout the zygotene and pachytene stages. Zygotene motion underlies the global tendency for colocalization of...

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Autores principales: Wanat, Jennifer J., Kim, Keun P., Koszul, Romain, Zanders, Sarah, Weiner, Beth, Kleckner, Nancy, Alani, Eric
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533701/
https://www.ncbi.nlm.nih.gov/pubmed/18818741
http://dx.doi.org/10.1371/journal.pgen.1000188
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author Wanat, Jennifer J.
Kim, Keun P.
Koszul, Romain
Zanders, Sarah
Weiner, Beth
Kleckner, Nancy
Alani, Eric
author_facet Wanat, Jennifer J.
Kim, Keun P.
Koszul, Romain
Zanders, Sarah
Weiner, Beth
Kleckner, Nancy
Alani, Eric
author_sort Wanat, Jennifer J.
collection PubMed
description Chromosome movements are a general feature of mid-prophase of meiosis. In budding yeast, meiotic chromosomes exhibit dynamic movements, led by nuclear envelope (NE)-associated telomeres, throughout the zygotene and pachytene stages. Zygotene motion underlies the global tendency for colocalization of NE-associated chromosome ends in a “bouquet.” In this study, we identify Csm4 as a new molecular participant in these processes and show that, unlike the two previously identified components, Ndj1 and Mps3, Csm4 is not required for meiosis-specific telomere/NE association. Instead, it acts to couple telomere/NE ensembles to a force generation mechanism. Mutants lacking Csm4 and/or Ndj1 display the following closely related phenotypes: (i) elevated crossover (CO) frequencies and decreased CO interference without abrogation of normal pathways; (ii) delayed progression of recombination, and recombination-coupled chromosome morphogenesis, with resulting delays in the MI division; and (iii) nondisjunction of homologs at the MI division for some reason other than absence of (the obligatory) CO(s). The recombination effects are discussed in the context of a model where the underlying defect is chromosome movement, the absence of which results in persistence of inappropriate chromosome relationships that, in turn, results in the observed mutant phenotypes.
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spelling pubmed-25337012008-09-26 Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis Wanat, Jennifer J. Kim, Keun P. Koszul, Romain Zanders, Sarah Weiner, Beth Kleckner, Nancy Alani, Eric PLoS Genet Research Article Chromosome movements are a general feature of mid-prophase of meiosis. In budding yeast, meiotic chromosomes exhibit dynamic movements, led by nuclear envelope (NE)-associated telomeres, throughout the zygotene and pachytene stages. Zygotene motion underlies the global tendency for colocalization of NE-associated chromosome ends in a “bouquet.” In this study, we identify Csm4 as a new molecular participant in these processes and show that, unlike the two previously identified components, Ndj1 and Mps3, Csm4 is not required for meiosis-specific telomere/NE association. Instead, it acts to couple telomere/NE ensembles to a force generation mechanism. Mutants lacking Csm4 and/or Ndj1 display the following closely related phenotypes: (i) elevated crossover (CO) frequencies and decreased CO interference without abrogation of normal pathways; (ii) delayed progression of recombination, and recombination-coupled chromosome morphogenesis, with resulting delays in the MI division; and (iii) nondisjunction of homologs at the MI division for some reason other than absence of (the obligatory) CO(s). The recombination effects are discussed in the context of a model where the underlying defect is chromosome movement, the absence of which results in persistence of inappropriate chromosome relationships that, in turn, results in the observed mutant phenotypes. Public Library of Science 2008-09-26 /pmc/articles/PMC2533701/ /pubmed/18818741 http://dx.doi.org/10.1371/journal.pgen.1000188 Text en Wanat et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wanat, Jennifer J.
Kim, Keun P.
Koszul, Romain
Zanders, Sarah
Weiner, Beth
Kleckner, Nancy
Alani, Eric
Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title_full Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title_fullStr Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title_full_unstemmed Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title_short Csm4, in Collaboration with Ndj1, Mediates Telomere-Led Chromosome Dynamics and Recombination during Yeast Meiosis
title_sort csm4, in collaboration with ndj1, mediates telomere-led chromosome dynamics and recombination during yeast meiosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533701/
https://www.ncbi.nlm.nih.gov/pubmed/18818741
http://dx.doi.org/10.1371/journal.pgen.1000188
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