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Orientation and structure of the Ndc80 complex on the microtubule lattice
The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microsc...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542468/ https://www.ncbi.nlm.nih.gov/pubmed/18794333 http://dx.doi.org/10.1083/jcb.200804170 |
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author | Wilson-Kubalek, Elizabeth M. Cheeseman, Iain M. Yoshioka, Craig Desai, Arshad Milligan, Ronald A. |
author_facet | Wilson-Kubalek, Elizabeth M. Cheeseman, Iain M. Yoshioka, Craig Desai, Arshad Milligan, Ronald A. |
author_sort | Wilson-Kubalek, Elizabeth M. |
collection | PubMed |
description | The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microscopy and helical image analysis to visualize the interaction of the Ndc80/Nuf2 dimer with microtubules. Our results, when combined with crystallography data, suggest that the globular domain of the Ndc80 subunit binds strongly at the interface between tubulin dimers and weakly at the adjacent intradimer interface along the protofilament axis. Such a binding mode, in which the Ndc80 complex interacts with sequential α/β-tubulin heterodimers, may be important for stabilizing kinetochore-bound microtubules. Additionally, we define the binding of the Ndc80 complex relative to microtubule polarity, which reveals that the microtubule interaction surface is at a considerable distance from the opposite kinetochore-anchored end; this binding geometry may facilitate polymerization and depolymerization at kinetochore-attached microtubule ends. |
format | Text |
id | pubmed-2542468 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25424682009-03-22 Orientation and structure of the Ndc80 complex on the microtubule lattice Wilson-Kubalek, Elizabeth M. Cheeseman, Iain M. Yoshioka, Craig Desai, Arshad Milligan, Ronald A. J Cell Biol Research Articles The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microscopy and helical image analysis to visualize the interaction of the Ndc80/Nuf2 dimer with microtubules. Our results, when combined with crystallography data, suggest that the globular domain of the Ndc80 subunit binds strongly at the interface between tubulin dimers and weakly at the adjacent intradimer interface along the protofilament axis. Such a binding mode, in which the Ndc80 complex interacts with sequential α/β-tubulin heterodimers, may be important for stabilizing kinetochore-bound microtubules. Additionally, we define the binding of the Ndc80 complex relative to microtubule polarity, which reveals that the microtubule interaction surface is at a considerable distance from the opposite kinetochore-anchored end; this binding geometry may facilitate polymerization and depolymerization at kinetochore-attached microtubule ends. The Rockefeller University Press 2008-09-22 /pmc/articles/PMC2542468/ /pubmed/18794333 http://dx.doi.org/10.1083/jcb.200804170 Text en © 2008 Wilson-Kubalek et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Wilson-Kubalek, Elizabeth M. Cheeseman, Iain M. Yoshioka, Craig Desai, Arshad Milligan, Ronald A. Orientation and structure of the Ndc80 complex on the microtubule lattice |
title | Orientation and structure of the Ndc80 complex on the microtubule lattice |
title_full | Orientation and structure of the Ndc80 complex on the microtubule lattice |
title_fullStr | Orientation and structure of the Ndc80 complex on the microtubule lattice |
title_full_unstemmed | Orientation and structure of the Ndc80 complex on the microtubule lattice |
title_short | Orientation and structure of the Ndc80 complex on the microtubule lattice |
title_sort | orientation and structure of the ndc80 complex on the microtubule lattice |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542468/ https://www.ncbi.nlm.nih.gov/pubmed/18794333 http://dx.doi.org/10.1083/jcb.200804170 |
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