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Orientation and structure of the Ndc80 complex on the microtubule lattice

The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microsc...

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Autores principales: Wilson-Kubalek, Elizabeth M., Cheeseman, Iain M., Yoshioka, Craig, Desai, Arshad, Milligan, Ronald A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542468/
https://www.ncbi.nlm.nih.gov/pubmed/18794333
http://dx.doi.org/10.1083/jcb.200804170
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author Wilson-Kubalek, Elizabeth M.
Cheeseman, Iain M.
Yoshioka, Craig
Desai, Arshad
Milligan, Ronald A.
author_facet Wilson-Kubalek, Elizabeth M.
Cheeseman, Iain M.
Yoshioka, Craig
Desai, Arshad
Milligan, Ronald A.
author_sort Wilson-Kubalek, Elizabeth M.
collection PubMed
description The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microscopy and helical image analysis to visualize the interaction of the Ndc80/Nuf2 dimer with microtubules. Our results, when combined with crystallography data, suggest that the globular domain of the Ndc80 subunit binds strongly at the interface between tubulin dimers and weakly at the adjacent intradimer interface along the protofilament axis. Such a binding mode, in which the Ndc80 complex interacts with sequential α/β-tubulin heterodimers, may be important for stabilizing kinetochore-bound microtubules. Additionally, we define the binding of the Ndc80 complex relative to microtubule polarity, which reveals that the microtubule interaction surface is at a considerable distance from the opposite kinetochore-anchored end; this binding geometry may facilitate polymerization and depolymerization at kinetochore-attached microtubule ends.
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spelling pubmed-25424682009-03-22 Orientation and structure of the Ndc80 complex on the microtubule lattice Wilson-Kubalek, Elizabeth M. Cheeseman, Iain M. Yoshioka, Craig Desai, Arshad Milligan, Ronald A. J Cell Biol Research Articles The four-subunit Ndc80 complex, comprised of Ndc80/Nuf2 and Spc24/Spc25 dimers, directly connects kinetochores to spindle microtubules. The complex is anchored to the kinetochore at the Spc24/25 end, and the Ndc80/Nuf2 dimer projects outward to bind to microtubules. Here, we use cryoelectron microscopy and helical image analysis to visualize the interaction of the Ndc80/Nuf2 dimer with microtubules. Our results, when combined with crystallography data, suggest that the globular domain of the Ndc80 subunit binds strongly at the interface between tubulin dimers and weakly at the adjacent intradimer interface along the protofilament axis. Such a binding mode, in which the Ndc80 complex interacts with sequential α/β-tubulin heterodimers, may be important for stabilizing kinetochore-bound microtubules. Additionally, we define the binding of the Ndc80 complex relative to microtubule polarity, which reveals that the microtubule interaction surface is at a considerable distance from the opposite kinetochore-anchored end; this binding geometry may facilitate polymerization and depolymerization at kinetochore-attached microtubule ends. The Rockefeller University Press 2008-09-22 /pmc/articles/PMC2542468/ /pubmed/18794333 http://dx.doi.org/10.1083/jcb.200804170 Text en © 2008 Wilson-Kubalek et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Wilson-Kubalek, Elizabeth M.
Cheeseman, Iain M.
Yoshioka, Craig
Desai, Arshad
Milligan, Ronald A.
Orientation and structure of the Ndc80 complex on the microtubule lattice
title Orientation and structure of the Ndc80 complex on the microtubule lattice
title_full Orientation and structure of the Ndc80 complex on the microtubule lattice
title_fullStr Orientation and structure of the Ndc80 complex on the microtubule lattice
title_full_unstemmed Orientation and structure of the Ndc80 complex on the microtubule lattice
title_short Orientation and structure of the Ndc80 complex on the microtubule lattice
title_sort orientation and structure of the ndc80 complex on the microtubule lattice
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542468/
https://www.ncbi.nlm.nih.gov/pubmed/18794333
http://dx.doi.org/10.1083/jcb.200804170
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