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Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542476/ https://www.ncbi.nlm.nih.gov/pubmed/18794328 http://dx.doi.org/10.1083/jcb.200712022 |
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author | Cai, Ting Wang, Haojie Chen, Yiliang Liu, Lijun Gunning, William T Quintas, Luis Eduardo M. Xie, Zi-Jian |
author_facet | Cai, Ting Wang, Haojie Chen, Yiliang Liu, Lijun Gunning, William T Quintas, Luis Eduardo M. Xie, Zi-Jian |
author_sort | Cai, Ting |
collection | PubMed |
description | Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 mediated by an N-terminal caveolin-binding motif within the ATPase α1 subunit. Moreover, knockdown of the Na/K-ATPase increases basal levels of active Src and stimulates endocytosis of Cav1 from the plasma membrane. Microtubule-dependent long-range directional trafficking in Na/K-ATPase–depleted cells results in perinuclear accumulation of Cav1-positive vesicles. Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. |
format | Text |
id | pubmed-2542476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25424762009-03-22 Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase Cai, Ting Wang, Haojie Chen, Yiliang Liu, Lijun Gunning, William T Quintas, Luis Eduardo M. Xie, Zi-Jian J Cell Biol Research Articles Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 mediated by an N-terminal caveolin-binding motif within the ATPase α1 subunit. Moreover, knockdown of the Na/K-ATPase increases basal levels of active Src and stimulates endocytosis of Cav1 from the plasma membrane. Microtubule-dependent long-range directional trafficking in Na/K-ATPase–depleted cells results in perinuclear accumulation of Cav1-positive vesicles. Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. The Rockefeller University Press 2008-09-22 /pmc/articles/PMC2542476/ /pubmed/18794328 http://dx.doi.org/10.1083/jcb.200712022 Text en © 2008 Cai et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Cai, Ting Wang, Haojie Chen, Yiliang Liu, Lijun Gunning, William T Quintas, Luis Eduardo M. Xie, Zi-Jian Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title | Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title_full | Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title_fullStr | Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title_full_unstemmed | Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title_short | Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase |
title_sort | regulation of caveolin-1 membrane trafficking by the na/k-atpase |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542476/ https://www.ncbi.nlm.nih.gov/pubmed/18794328 http://dx.doi.org/10.1083/jcb.200712022 |
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