Cargando…

Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase

Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the...

Descripción completa

Detalles Bibliográficos
Autores principales: Cai, Ting, Wang, Haojie, Chen, Yiliang, Liu, Lijun, Gunning, William T, Quintas, Luis Eduardo M., Xie, Zi-Jian
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542476/
https://www.ncbi.nlm.nih.gov/pubmed/18794328
http://dx.doi.org/10.1083/jcb.200712022
_version_ 1782159157356396544
author Cai, Ting
Wang, Haojie
Chen, Yiliang
Liu, Lijun
Gunning, William T
Quintas, Luis Eduardo M.
Xie, Zi-Jian
author_facet Cai, Ting
Wang, Haojie
Chen, Yiliang
Liu, Lijun
Gunning, William T
Quintas, Luis Eduardo M.
Xie, Zi-Jian
author_sort Cai, Ting
collection PubMed
description Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 mediated by an N-terminal caveolin-binding motif within the ATPase α1 subunit. Moreover, knockdown of the Na/K-ATPase increases basal levels of active Src and stimulates endocytosis of Cav1 from the plasma membrane. Microtubule-dependent long-range directional trafficking in Na/K-ATPase–depleted cells results in perinuclear accumulation of Cav1-positive vesicles. Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool.
format Text
id pubmed-2542476
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-25424762009-03-22 Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase Cai, Ting Wang, Haojie Chen, Yiliang Liu, Lijun Gunning, William T Quintas, Luis Eduardo M. Xie, Zi-Jian J Cell Biol Research Articles Here, we show that the Na/K-ATPase interacts with caveolin-1 (Cav1) and regulates Cav1 trafficking. Graded knockdown of Na/K-ATPase decreases the plasma membrane pool of Cav1, which results in a significant reduction in the number of caveolae on the cell surface. These effects are independent of the pumping function of Na/K-ATPase, and instead depend on interaction between Na/K-ATPase and Cav1 mediated by an N-terminal caveolin-binding motif within the ATPase α1 subunit. Moreover, knockdown of the Na/K-ATPase increases basal levels of active Src and stimulates endocytosis of Cav1 from the plasma membrane. Microtubule-dependent long-range directional trafficking in Na/K-ATPase–depleted cells results in perinuclear accumulation of Cav1-positive vesicles. Finally, Na/K-ATPase knockdown has no effect on processing or exit of Cav1 from the Golgi. Thus, the Na/K-ATPase regulates Cav1 endocytic trafficking and stabilizes the Cav1 plasma membrane pool. The Rockefeller University Press 2008-09-22 /pmc/articles/PMC2542476/ /pubmed/18794328 http://dx.doi.org/10.1083/jcb.200712022 Text en © 2008 Cai et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Cai, Ting
Wang, Haojie
Chen, Yiliang
Liu, Lijun
Gunning, William T
Quintas, Luis Eduardo M.
Xie, Zi-Jian
Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title_full Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title_fullStr Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title_full_unstemmed Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title_short Regulation of caveolin-1 membrane trafficking by the Na/K-ATPase
title_sort regulation of caveolin-1 membrane trafficking by the na/k-atpase
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542476/
https://www.ncbi.nlm.nih.gov/pubmed/18794328
http://dx.doi.org/10.1083/jcb.200712022
work_keys_str_mv AT caiting regulationofcaveolin1membranetraffickingbythenakatpase
AT wanghaojie regulationofcaveolin1membranetraffickingbythenakatpase
AT chenyiliang regulationofcaveolin1membranetraffickingbythenakatpase
AT liulijun regulationofcaveolin1membranetraffickingbythenakatpase
AT gunningwilliamt regulationofcaveolin1membranetraffickingbythenakatpase
AT quintasluiseduardom regulationofcaveolin1membranetraffickingbythenakatpase
AT xiezijian regulationofcaveolin1membranetraffickingbythenakatpase