The relative test performance characteristics of two commercial assays for the detection of Mycobacterium tuberculosis complex in paraffin-fixed human biopsy specimens

The Seeplex™ TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specime...

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Detalles Bibliográficos
Autores principales: Drews, Steven J, Eshaghi, AliReza, Pyskir, Daria, Chedore, Pam, Lombos, Ernesto, Broukhanski, George, Higgins, Rachel, Fisman, David N, Blair, Joanne, Jamieson, Frances
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2546368/
https://www.ncbi.nlm.nih.gov/pubmed/18778465
http://dx.doi.org/10.1186/1746-1596-3-37
Descripción
Sumario:The Seeplex™ TB Detection-2 assay (Rockville, MD) is a nested endpoint PCR for the Mycobacterium tuberculosis complex (MTBC) targets IS6110 and MPB64 that utilizes dual priming oligonucleotide technology. When used to detect the presence of MTBC DNA in formalin-fixed paraffin-embedded tissue specimens, the sensitivity and specificity of this assay is equivalent to a labor-intensive traditional endpoint PCR assay and is more sensitive than a commercial real-time PCR assay.

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