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Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume

BACKGROUND: Welding fume has been categorized as "possibly carcinogenic" to humans. Our objectives were to characterize the lung response to carcinogenic and non-carcinogenic metal-containing welding fumes and to determine if these fumes caused increased lung tumorigenicity in A/J mice, a...

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Autores principales: Zeidler-Erdely, Patti C, Kashon, Michael L, Battelli, Lori A, Young, Shih-Houng, Erdely, Aaron, Roberts, Jenny R, Reynolds, Steven H, Antonini, James M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2546436/
https://www.ncbi.nlm.nih.gov/pubmed/18778475
http://dx.doi.org/10.1186/1743-8977-5-12
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author Zeidler-Erdely, Patti C
Kashon, Michael L
Battelli, Lori A
Young, Shih-Houng
Erdely, Aaron
Roberts, Jenny R
Reynolds, Steven H
Antonini, James M
author_facet Zeidler-Erdely, Patti C
Kashon, Michael L
Battelli, Lori A
Young, Shih-Houng
Erdely, Aaron
Roberts, Jenny R
Reynolds, Steven H
Antonini, James M
author_sort Zeidler-Erdely, Patti C
collection PubMed
description BACKGROUND: Welding fume has been categorized as "possibly carcinogenic" to humans. Our objectives were to characterize the lung response to carcinogenic and non-carcinogenic metal-containing welding fumes and to determine if these fumes caused increased lung tumorigenicity in A/J mice, a lung tumor susceptible strain. We exposed male A/J and C57BL/6J, a lung tumor resistant strain, by pharyngeal aspiration four times (once every 3 days) to 85 μg of gas metal arc-mild steel (GMA-MS), GMA-stainless steel (SS), or manual metal arc-SS (MMA-SS) fume, or to 25.5 μg soluble hexavalent chromium (S-Cr). Shams were exposed to saline vehicle. Bronchoalveolar lavage (BAL) was done at 2, 7, and 28 days post-exposure. For the lung tumor study, gross tumor counts and histopathological changes were assessed in A/J mice at 48 and 78 weeks post-exposure. RESULTS: BAL revealed notable strain-dependent differences with regards to the degree and resolution of the inflammatory response after exposure to the fumes. At 48 weeks, carcinogenic metal-containing GMA-SS fume caused the greatest increase in tumor multiplicity and incidence, but this was not different from sham. By 78 weeks, tumor incidence in the GMA-SS group versus sham approached significance (p = 0.057). A significant increase in perivascular/peribronchial lymphoid infiltrates for the GMA-SS group versus sham and an increased persistence of this fume in lung cells compared to the other welding fumes was found. CONCLUSION: The increased persistence of GMA-SS fume in combination with its metal composition may trigger a chronic, but mild, inflammatory state in the lung possibly enhancing tumorigenesis in this susceptible mouse strain.
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spelling pubmed-25464362008-09-20 Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume Zeidler-Erdely, Patti C Kashon, Michael L Battelli, Lori A Young, Shih-Houng Erdely, Aaron Roberts, Jenny R Reynolds, Steven H Antonini, James M Part Fibre Toxicol Research BACKGROUND: Welding fume has been categorized as "possibly carcinogenic" to humans. Our objectives were to characterize the lung response to carcinogenic and non-carcinogenic metal-containing welding fumes and to determine if these fumes caused increased lung tumorigenicity in A/J mice, a lung tumor susceptible strain. We exposed male A/J and C57BL/6J, a lung tumor resistant strain, by pharyngeal aspiration four times (once every 3 days) to 85 μg of gas metal arc-mild steel (GMA-MS), GMA-stainless steel (SS), or manual metal arc-SS (MMA-SS) fume, or to 25.5 μg soluble hexavalent chromium (S-Cr). Shams were exposed to saline vehicle. Bronchoalveolar lavage (BAL) was done at 2, 7, and 28 days post-exposure. For the lung tumor study, gross tumor counts and histopathological changes were assessed in A/J mice at 48 and 78 weeks post-exposure. RESULTS: BAL revealed notable strain-dependent differences with regards to the degree and resolution of the inflammatory response after exposure to the fumes. At 48 weeks, carcinogenic metal-containing GMA-SS fume caused the greatest increase in tumor multiplicity and incidence, but this was not different from sham. By 78 weeks, tumor incidence in the GMA-SS group versus sham approached significance (p = 0.057). A significant increase in perivascular/peribronchial lymphoid infiltrates for the GMA-SS group versus sham and an increased persistence of this fume in lung cells compared to the other welding fumes was found. CONCLUSION: The increased persistence of GMA-SS fume in combination with its metal composition may trigger a chronic, but mild, inflammatory state in the lung possibly enhancing tumorigenesis in this susceptible mouse strain. BioMed Central 2008-09-08 /pmc/articles/PMC2546436/ /pubmed/18778475 http://dx.doi.org/10.1186/1743-8977-5-12 Text en Copyright © 2008 Zeidler-Erdely et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zeidler-Erdely, Patti C
Kashon, Michael L
Battelli, Lori A
Young, Shih-Houng
Erdely, Aaron
Roberts, Jenny R
Reynolds, Steven H
Antonini, James M
Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title_full Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title_fullStr Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title_full_unstemmed Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title_short Pulmonary inflammation and tumor induction in lung tumor susceptible A/J and resistant C57BL/6J mice exposed to welding fume
title_sort pulmonary inflammation and tumor induction in lung tumor susceptible a/j and resistant c57bl/6j mice exposed to welding fume
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2546436/
https://www.ncbi.nlm.nih.gov/pubmed/18778475
http://dx.doi.org/10.1186/1743-8977-5-12
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