Automated HPLC monitoring of broth components on bioreactors

Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtr...

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Detalles Bibliográficos
Autores principales: Favre, Eric, Pugeaud, Patrick, Raboud, Jean Philippe, Péringer, Paul
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547807/
https://www.ncbi.nlm.nih.gov/pubmed/18925256
http://dx.doi.org/10.1155/S1463924689000532
Descripción
Sumario:Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtrate stream has been successfully connected to a bioreactor in order to perform the automated HPLC analysis of broth components. The monitoring of the carbon source (lactose), and minor products (glycerol, acetate and succinate) during a yeast culture (Kluyveromyces marxianus) is shown.

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