Automated HPLC monitoring of broth components on bioreactors

Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtr...

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Detalles Bibliográficos
Autores principales: Favre, Eric, Pugeaud, Patrick, Raboud, Jean Philippe, Péringer, Paul
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1989
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547807/
https://www.ncbi.nlm.nih.gov/pubmed/18925256
http://dx.doi.org/10.1155/S1463924689000532
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author Favre, Eric
Pugeaud, Patrick
Raboud, Jean Philippe
Péringer, Paul
author_facet Favre, Eric
Pugeaud, Patrick
Raboud, Jean Philippe
Péringer, Paul
author_sort Favre, Eric
collection PubMed
description Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtrate stream has been successfully connected to a bioreactor in order to perform the automated HPLC analysis of broth components. The monitoring of the carbon source (lactose), and minor products (glycerol, acetate and succinate) during a yeast culture (Kluyveromyces marxianus) is shown.
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spelling pubmed-25478072008-10-16 Automated HPLC monitoring of broth components on bioreactors Favre, Eric Pugeaud, Patrick Raboud, Jean Philippe Péringer, Paul J Automat Chem Research Article Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtrate stream has been successfully connected to a bioreactor in order to perform the automated HPLC analysis of broth components. The monitoring of the carbon source (lactose), and minor products (glycerol, acetate and succinate) during a yeast culture (Kluyveromyces marxianus) is shown. Hindawi Publishing Corporation 1989 /pmc/articles/PMC2547807/ /pubmed/18925256 http://dx.doi.org/10.1155/S1463924689000532 Text en Copyright © 1989 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Favre, Eric
Pugeaud, Patrick
Raboud, Jean Philippe
Péringer, Paul
Automated HPLC monitoring of broth components on bioreactors
title Automated HPLC monitoring of broth components on bioreactors
title_full Automated HPLC monitoring of broth components on bioreactors
title_fullStr Automated HPLC monitoring of broth components on bioreactors
title_full_unstemmed Automated HPLC monitoring of broth components on bioreactors
title_short Automated HPLC monitoring of broth components on bioreactors
title_sort automated hplc monitoring of broth components on bioreactors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547807/
https://www.ncbi.nlm.nih.gov/pubmed/18925256
http://dx.doi.org/10.1155/S1463924689000532
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AT peringerpaul automatedhplcmonitoringofbrothcomponentsonbioreactors

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