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Evaluation of the Dacos 3.0 analyser
The selective multitest Coulter Dacos 3.0 analyser was evaluated according to the guidelines of the Comisión de Instrumentación de la Sociedad Española de Química Clínica and of the European Committee for Clinical Laboratory Standards. The evaluation was performed in four steps: examination of the a...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
1990
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547829/ https://www.ncbi.nlm.nih.gov/pubmed/18925270 http://dx.doi.org/10.1155/S1463924690000207 |
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author | Pons, Joan Farré Alumá, Alba Antoja, Felipe Biosca, Carmen Alsina, María-Jesús Galimany, Román |
author_facet | Pons, Joan Farré Alumá, Alba Antoja, Felipe Biosca, Carmen Alsina, María-Jesús Galimany, Román |
author_sort | Pons, Joan Farré |
collection | PubMed |
description | The selective multitest Coulter Dacos 3.0 analyser was evaluated according to the guidelines of the Comisión de Instrumentación de la Sociedad Española de Química Clínica and of the European Committee for Clinical Laboratory Standards. The evaluation was performed in four steps: examination of the analytical units; evaluation of routine working; study of interferences; and assessment of practicability. The evaluation included a photometric study. The inaccuracy is acceptable for 340 nm and 420 nm, and the imprecision at absorbances from 0.05 to 2.00 ranged from 0.06 to 0.28% at 340 nm and from 0.06 to 0.08% at 420 nm. The linearity showed some dispersion at low absorbance for PNP at 420 nm and the drift was negligible. The imprecision of the pipette delivery system, the temperature control system and the washing system were satisfactory. In routine work conditions, seven analytical methods were studied: glucose, creatinine, iron, total protein, AST, ALP and calcium. Within-run imprecision ranged, at low concentrations, from 0.9% (CV) for glucose, to 7.6% (CV) for iron; at medium concentrations, from 0.7% (CV) for total protein to 5.2% (CV) to creatinine; and at high concentrations, it ranged from 0.6% (CV) for glucose to 3.9% (CV) for ALP. Between-run imprecision at low concentrations ranged from 1.4% (CV) for glucose to 15.1% (CV) for iron; at medium concentrations it ranged from 1.2% (CV) for protein to 6.7% (CV) for iron; and at high concentrations the range is from l.2for AST to 5.7% (CV) for iron. No contamination was found in the sample carry-over study. Some contamination was found in the reagent carry-over study (total protein due to iron and calcium reagents). Relative inaccuracy is good for all the constituents assayed. Only LDH (high and low levels) and urate (low level) showed weak and negative interference caused by turbidity, and γ-GT (high level) and amylase, bilirubin and ALP (two levels) showed a negative interference caused by haemolysis. |
format | Text |
id | pubmed-2547829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1990 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-25478292008-10-16 Evaluation of the Dacos 3.0 analyser Pons, Joan Farré Alumá, Alba Antoja, Felipe Biosca, Carmen Alsina, María-Jesús Galimany, Román J Automat Chem Research Article The selective multitest Coulter Dacos 3.0 analyser was evaluated according to the guidelines of the Comisión de Instrumentación de la Sociedad Española de Química Clínica and of the European Committee for Clinical Laboratory Standards. The evaluation was performed in four steps: examination of the analytical units; evaluation of routine working; study of interferences; and assessment of practicability. The evaluation included a photometric study. The inaccuracy is acceptable for 340 nm and 420 nm, and the imprecision at absorbances from 0.05 to 2.00 ranged from 0.06 to 0.28% at 340 nm and from 0.06 to 0.08% at 420 nm. The linearity showed some dispersion at low absorbance for PNP at 420 nm and the drift was negligible. The imprecision of the pipette delivery system, the temperature control system and the washing system were satisfactory. In routine work conditions, seven analytical methods were studied: glucose, creatinine, iron, total protein, AST, ALP and calcium. Within-run imprecision ranged, at low concentrations, from 0.9% (CV) for glucose, to 7.6% (CV) for iron; at medium concentrations, from 0.7% (CV) for total protein to 5.2% (CV) to creatinine; and at high concentrations, it ranged from 0.6% (CV) for glucose to 3.9% (CV) for ALP. Between-run imprecision at low concentrations ranged from 1.4% (CV) for glucose to 15.1% (CV) for iron; at medium concentrations it ranged from 1.2% (CV) for protein to 6.7% (CV) for iron; and at high concentrations the range is from l.2for AST to 5.7% (CV) for iron. No contamination was found in the sample carry-over study. Some contamination was found in the reagent carry-over study (total protein due to iron and calcium reagents). Relative inaccuracy is good for all the constituents assayed. Only LDH (high and low levels) and urate (low level) showed weak and negative interference caused by turbidity, and γ-GT (high level) and amylase, bilirubin and ALP (two levels) showed a negative interference caused by haemolysis. Hindawi Publishing Corporation 1990 /pmc/articles/PMC2547829/ /pubmed/18925270 http://dx.doi.org/10.1155/S1463924690000207 Text en Copyright © 1990 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Pons, Joan Farré Alumá, Alba Antoja, Felipe Biosca, Carmen Alsina, María-Jesús Galimany, Román Evaluation of the Dacos 3.0 analyser |
title | Evaluation of the Dacos 3.0 analyser |
title_full | Evaluation of the Dacos 3.0 analyser |
title_fullStr | Evaluation of the Dacos 3.0 analyser |
title_full_unstemmed | Evaluation of the Dacos 3.0 analyser |
title_short | Evaluation of the Dacos 3.0 analyser |
title_sort | evaluation of the dacos 3.0 analyser |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2547829/ https://www.ncbi.nlm.nih.gov/pubmed/18925270 http://dx.doi.org/10.1155/S1463924690000207 |
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