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Development and analytical performance of an automated screening method for cannabinoids on the Dimension clinical chemistry system
A fully automated, random access method for the determination of cannabinoids (UTHC) was developed for the Dimension AR and XL clinical chemistry systems. The method utilizes Abuscreen ONLINE reagents and a multianalyte liquid calibrator containing 11-nor-Δ(9)-THC-9-carboxylic acid. Within-run and t...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
1997
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548125/ https://www.ncbi.nlm.nih.gov/pubmed/18924804 http://dx.doi.org/10.1155/S1463924697000187 |
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author | Obzansky, D. M. Gorman, E. G. Kramer, S. P. Masulli, I. S. Nuzzaci, E. A. Skogen, W. F. |
author_facet | Obzansky, D. M. Gorman, E. G. Kramer, S. P. Masulli, I. S. Nuzzaci, E. A. Skogen, W. F. |
author_sort | Obzansky, D. M. |
collection | PubMed |
description | A fully automated, random access method for the determination of cannabinoids (UTHC) was developed for the Dimension AR and XL clinical chemistry systems. The method utilizes Abuscreen ONLINE reagents and a multianalyte liquid calibrator containing 11-nor-Δ(9)-THC-9-carboxylic acid. Within-run and total reproducibility, determined using NCCLS protocol EP5- T2, was less than 0.6% and 1.6% CV, respectively, at all concentrations. Calibration stability was retained for at least 30 days. An extensive evaluation of non-structurally related drugs and various physiological substances indicated lack of interference in the method. No sample carry-over was observed following a specimen containing 1886 ng/ml 11-nor-Δ(9)-THC-9-carboxylic acid. A 99.1% agreement (N = 445 samples) was found between an EMIT based method on the aca discrete clinical analyser and the Dimension UTHC method. Dimension clinical chemistry system and aca discrete clinical analyser are registered trademarks of Dade International. |
format | Text |
id | pubmed-2548125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1997 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-25481252008-10-16 Development and analytical performance of an automated screening method for cannabinoids on the Dimension clinical chemistry system Obzansky, D. M. Gorman, E. G. Kramer, S. P. Masulli, I. S. Nuzzaci, E. A. Skogen, W. F. J Automat Chem Research Article A fully automated, random access method for the determination of cannabinoids (UTHC) was developed for the Dimension AR and XL clinical chemistry systems. The method utilizes Abuscreen ONLINE reagents and a multianalyte liquid calibrator containing 11-nor-Δ(9)-THC-9-carboxylic acid. Within-run and total reproducibility, determined using NCCLS protocol EP5- T2, was less than 0.6% and 1.6% CV, respectively, at all concentrations. Calibration stability was retained for at least 30 days. An extensive evaluation of non-structurally related drugs and various physiological substances indicated lack of interference in the method. No sample carry-over was observed following a specimen containing 1886 ng/ml 11-nor-Δ(9)-THC-9-carboxylic acid. A 99.1% agreement (N = 445 samples) was found between an EMIT based method on the aca discrete clinical analyser and the Dimension UTHC method. Dimension clinical chemistry system and aca discrete clinical analyser are registered trademarks of Dade International. Hindawi Publishing Corporation 1997 /pmc/articles/PMC2548125/ /pubmed/18924804 http://dx.doi.org/10.1155/S1463924697000187 Text en Copyright © 1997 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Obzansky, D. M. Gorman, E. G. Kramer, S. P. Masulli, I. S. Nuzzaci, E. A. Skogen, W. F. Development and analytical performance of an automated screening method for cannabinoids on the Dimension clinical chemistry system |
title | Development and analytical performance of an automated screening method for
cannabinoids on the Dimension clinical chemistry system |
title_full | Development and analytical performance of an automated screening method for
cannabinoids on the Dimension clinical chemistry system |
title_fullStr | Development and analytical performance of an automated screening method for
cannabinoids on the Dimension clinical chemistry system |
title_full_unstemmed | Development and analytical performance of an automated screening method for
cannabinoids on the Dimension clinical chemistry system |
title_short | Development and analytical performance of an automated screening method for
cannabinoids on the Dimension clinical chemistry system |
title_sort | development and analytical performance of an automated screening method for
cannabinoids on the dimension clinical chemistry system |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548125/ https://www.ncbi.nlm.nih.gov/pubmed/18924804 http://dx.doi.org/10.1155/S1463924697000187 |
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