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Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique

An apparatus that operates with an isotope-ratio mass spectrometer to automatically perform nitrogen isotope analyses by the Rittenberg technique was modified to permit the use of nitrous oxide (N(2)O) instead of Freon (CCl(2)F(2) or CHClF(2)) for the purging of air prior to hypobromite oxidation of...

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Detalles Bibliográficos
Autores principales: Mulvaney, R. L., Khan, S. A., Sims, G. K., Stevens, W. B.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548131/
https://www.ncbi.nlm.nih.gov/pubmed/18924803
http://dx.doi.org/10.1155/S1463924697000175
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author Mulvaney, R. L.
Khan, S. A.
Sims, G. K.
Stevens, W. B.
author_facet Mulvaney, R. L.
Khan, S. A.
Sims, G. K.
Stevens, W. B.
author_sort Mulvaney, R. L.
collection PubMed
description An apparatus that operates with an isotope-ratio mass spectrometer to automatically perform nitrogen isotope analyses by the Rittenberg technique was modified to permit the use of nitrous oxide (N(2)O) instead of Freon (CCl(2)F(2) or CHClF(2)) for the purging of air prior to hypobromite oxidation of ammonium-N to N(2) in a plastic microplate. Analytical performance was unaffected by the modifications. Up to 768 samples can be processed in a single loading, at a rate of 6 to 12 samples/h. Within the range of 0.2 to 20 atom % (15)N, isotope-ratio analyses of 50 to 200 μg of N using the automated Rittenberg apparatus (ARA) with a double-collector mass spectrometer were accurate to within 0.7%, as compared to manual Rittenberg analyses of 1 mg of N using the same mass spectrometer with a dual-inlet system. Automated analyses of 20μg of N were accurate to within 2%, and automated analyses of 10 μg of N were accurate to within 7%. The relative standard deviation for measurements at the natural abundance level (10 analyses, 20-200 μg of N) was < 0.04 %.
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spelling pubmed-25481312008-10-16 Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique Mulvaney, R. L. Khan, S. A. Sims, G. K. Stevens, W. B. J Automat Chem Research Article An apparatus that operates with an isotope-ratio mass spectrometer to automatically perform nitrogen isotope analyses by the Rittenberg technique was modified to permit the use of nitrous oxide (N(2)O) instead of Freon (CCl(2)F(2) or CHClF(2)) for the purging of air prior to hypobromite oxidation of ammonium-N to N(2) in a plastic microplate. Analytical performance was unaffected by the modifications. Up to 768 samples can be processed in a single loading, at a rate of 6 to 12 samples/h. Within the range of 0.2 to 20 atom % (15)N, isotope-ratio analyses of 50 to 200 μg of N using the automated Rittenberg apparatus (ARA) with a double-collector mass spectrometer were accurate to within 0.7%, as compared to manual Rittenberg analyses of 1 mg of N using the same mass spectrometer with a dual-inlet system. Automated analyses of 20μg of N were accurate to within 2%, and automated analyses of 10 μg of N were accurate to within 7%. The relative standard deviation for measurements at the natural abundance level (10 analyses, 20-200 μg of N) was < 0.04 %. Hindawi Publishing Corporation 1997 /pmc/articles/PMC2548131/ /pubmed/18924803 http://dx.doi.org/10.1155/S1463924697000175 Text en Copyright © 1997 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mulvaney, R. L.
Khan, S. A.
Sims, G. K.
Stevens, W. B.
Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title_full Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title_fullStr Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title_full_unstemmed Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title_short Use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the Rittenberg technique
title_sort use of nitrous oxide as a purge gas for automated nitrogen isotope analysis by the rittenberg technique
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548131/
https://www.ncbi.nlm.nih.gov/pubmed/18924803
http://dx.doi.org/10.1155/S1463924697000175
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