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Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria
Human Immunodeficiency Virus (HIV) protease initiates apoptosis of HIV-infected cells by proteolytic cleavage of procaspase 8, creating a novel peptide termed casp8p41. Expression of casp8p41 alone is sufficient to initiate caspase-dependent cell death associated with mitochondrial depolarization. S...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Bentham Science Publishers Ltd.
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548302/ https://www.ncbi.nlm.nih.gov/pubmed/18818773 http://dx.doi.org/10.2174/1874357900701010039 |
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author | Algeciras-Schimnich, Alicia Belzacq-Casagrande, Anne-Sophie Bren, Gary D Nie, Zilin Taylor, Julie A Rizza, Stacey A Brenner, Catherine Badley, Andrew D |
author_facet | Algeciras-Schimnich, Alicia Belzacq-Casagrande, Anne-Sophie Bren, Gary D Nie, Zilin Taylor, Julie A Rizza, Stacey A Brenner, Catherine Badley, Andrew D |
author_sort | Algeciras-Schimnich, Alicia |
collection | PubMed |
description | Human Immunodeficiency Virus (HIV) protease initiates apoptosis of HIV-infected cells by proteolytic cleavage of procaspase 8, creating a novel peptide termed casp8p41. Expression of casp8p41 alone is sufficient to initiate caspase-dependent cell death associated with mitochondrial depolarization. Since casp8p41 does not contain the catalytic cysteine at position 360, the mechanism by which casp8p41 initiates apoptosis is unclear. We demonstrate that casp8p41 directly causes mitochondrial depolarization and release of cytochrome c with downstream caspase 9 activation. Moreover, death induced by casp8p41 requires the presence of mitochondria, and in intact cells, casp8p41 colocalizes with mitochondria. These results illuminate a novel mechanism of cell death induced by a caspase 8 cleavage fragment whereby mitochondrial interaction leads to depolarization and cytochrome c release. |
format | Text |
id | pubmed-2548302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Bentham Science Publishers Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-25483022008-09-23 Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria Algeciras-Schimnich, Alicia Belzacq-Casagrande, Anne-Sophie Bren, Gary D Nie, Zilin Taylor, Julie A Rizza, Stacey A Brenner, Catherine Badley, Andrew D Open Virol J Article Human Immunodeficiency Virus (HIV) protease initiates apoptosis of HIV-infected cells by proteolytic cleavage of procaspase 8, creating a novel peptide termed casp8p41. Expression of casp8p41 alone is sufficient to initiate caspase-dependent cell death associated with mitochondrial depolarization. Since casp8p41 does not contain the catalytic cysteine at position 360, the mechanism by which casp8p41 initiates apoptosis is unclear. We demonstrate that casp8p41 directly causes mitochondrial depolarization and release of cytochrome c with downstream caspase 9 activation. Moreover, death induced by casp8p41 requires the presence of mitochondria, and in intact cells, casp8p41 colocalizes with mitochondria. These results illuminate a novel mechanism of cell death induced by a caspase 8 cleavage fragment whereby mitochondrial interaction leads to depolarization and cytochrome c release. Bentham Science Publishers Ltd. 2007-12-27 /pmc/articles/PMC2548302/ /pubmed/18818773 http://dx.doi.org/10.2174/1874357900701010039 Text en 2007 Bentham Science Publishers Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Algeciras-Schimnich, Alicia Belzacq-Casagrande, Anne-Sophie Bren, Gary D Nie, Zilin Taylor, Julie A Rizza, Stacey A Brenner, Catherine Badley, Andrew D Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title | Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title_full | Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title_fullStr | Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title_full_unstemmed | Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title_short | Analysis of HIV Protease Killing Through Caspase 8 Reveals a Novel Interaction Between Caspase 8 and Mitochondria |
title_sort | analysis of hiv protease killing through caspase 8 reveals a novel interaction between caspase 8 and mitochondria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2548302/ https://www.ncbi.nlm.nih.gov/pubmed/18818773 http://dx.doi.org/10.2174/1874357900701010039 |
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