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ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction

The effect of intracellular adenosine triphosphate (ATP) on the “common gating” of the CLC-1 chloride channel has been studied by several laboratories with controversial results. Our previous study on the channel expressed in Xenopus oocytes using excised inside-out patch-clamp methods showed a robu...

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Autores principales: Zhang, Xiao-Dong, Tseng, Pang-Yen, Chen, Tsung-Yu
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553389/
https://www.ncbi.nlm.nih.gov/pubmed/18824589
http://dx.doi.org/10.1085/jgp.200810023
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author Zhang, Xiao-Dong
Tseng, Pang-Yen
Chen, Tsung-Yu
author_facet Zhang, Xiao-Dong
Tseng, Pang-Yen
Chen, Tsung-Yu
author_sort Zhang, Xiao-Dong
collection PubMed
description The effect of intracellular adenosine triphosphate (ATP) on the “common gating” of the CLC-1 chloride channel has been studied by several laboratories with controversial results. Our previous study on the channel expressed in Xenopus oocytes using excised inside-out patch-clamp methods showed a robust effect of ATP in shifting the open probability curve of the common gate toward more depolarizing voltages (Tseng, P.Y., B. Bennetts, and T.Y. Chen. 2007. J. Gen. Physiol. 130:217–221). The results were consistent with those from studying the channel expressed in mammalian cells using whole cell recording methods (Bennetts, B., M.W. Parker, and B.A. Cromer. 2007. J. Biol. Chem. 282:32780–32791). However, a recent study using excised-patch recording methods for channels expressed in Xenopus oocytes reported that ATP had no direct effect on CLC-1 (Zifarelli, G., and M. Pusch. 2008. J. Gen. Physiol. 131:109–116). Here, we report that oxidation of CLC-1 may be the culprit underlying the controversy. When patches were excised from mammalian cells, the sensitivity to ATP was lost quickly—within 2–3 min. This loss of ATP sensitivity could be prevented or reversed by reducing agents. On the other hand, CLC-1 expressed in Xenopus oocytes lost the ATP sensitivity when patches were treated with oxidizing reagents. These results suggest a novel view in muscle physiology that the mechanisms controlling muscle fatigability may include the oxidation of CLC-1.
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spelling pubmed-25533892009-04-01 ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction Zhang, Xiao-Dong Tseng, Pang-Yen Chen, Tsung-Yu J Gen Physiol Articles The effect of intracellular adenosine triphosphate (ATP) on the “common gating” of the CLC-1 chloride channel has been studied by several laboratories with controversial results. Our previous study on the channel expressed in Xenopus oocytes using excised inside-out patch-clamp methods showed a robust effect of ATP in shifting the open probability curve of the common gate toward more depolarizing voltages (Tseng, P.Y., B. Bennetts, and T.Y. Chen. 2007. J. Gen. Physiol. 130:217–221). The results were consistent with those from studying the channel expressed in mammalian cells using whole cell recording methods (Bennetts, B., M.W. Parker, and B.A. Cromer. 2007. J. Biol. Chem. 282:32780–32791). However, a recent study using excised-patch recording methods for channels expressed in Xenopus oocytes reported that ATP had no direct effect on CLC-1 (Zifarelli, G., and M. Pusch. 2008. J. Gen. Physiol. 131:109–116). Here, we report that oxidation of CLC-1 may be the culprit underlying the controversy. When patches were excised from mammalian cells, the sensitivity to ATP was lost quickly—within 2–3 min. This loss of ATP sensitivity could be prevented or reversed by reducing agents. On the other hand, CLC-1 expressed in Xenopus oocytes lost the ATP sensitivity when patches were treated with oxidizing reagents. These results suggest a novel view in muscle physiology that the mechanisms controlling muscle fatigability may include the oxidation of CLC-1. The Rockefeller University Press 2008-10 /pmc/articles/PMC2553389/ /pubmed/18824589 http://dx.doi.org/10.1085/jgp.200810023 Text en © 2008 Zhang et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jgp.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Articles
Zhang, Xiao-Dong
Tseng, Pang-Yen
Chen, Tsung-Yu
ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title_full ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title_fullStr ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title_full_unstemmed ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title_short ATP Inhibition of CLC-1 Is Controlled by Oxidation and Reduction
title_sort atp inhibition of clc-1 is controlled by oxidation and reduction
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553389/
https://www.ncbi.nlm.nih.gov/pubmed/18824589
http://dx.doi.org/10.1085/jgp.200810023
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