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Kinetics of charge transfer in DNA containing a mismatch

Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains...

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Autores principales: Osakada, Yasuko, Kawai, Kiyohiko, Fujitsuka, Mamoru, Majima, Tetsuro
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553589/
https://www.ncbi.nlm.nih.gov/pubmed/18757889
http://dx.doi.org/10.1093/nar/gkn505
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author Osakada, Yasuko
Kawai, Kiyohiko
Fujitsuka, Mamoru
Majima, Tetsuro
author_facet Osakada, Yasuko
Kawai, Kiyohiko
Fujitsuka, Mamoru
Majima, Tetsuro
author_sort Osakada, Yasuko
collection PubMed
description Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch.
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spelling pubmed-25535892008-10-01 Kinetics of charge transfer in DNA containing a mismatch Osakada, Yasuko Kawai, Kiyohiko Fujitsuka, Mamoru Majima, Tetsuro Nucleic Acids Res Chemistry and Synthetic Biology Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch. Oxford University Press 2008-10 2008-08-30 /pmc/articles/PMC2553589/ /pubmed/18757889 http://dx.doi.org/10.1093/nar/gkn505 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry and Synthetic Biology
Osakada, Yasuko
Kawai, Kiyohiko
Fujitsuka, Mamoru
Majima, Tetsuro
Kinetics of charge transfer in DNA containing a mismatch
title Kinetics of charge transfer in DNA containing a mismatch
title_full Kinetics of charge transfer in DNA containing a mismatch
title_fullStr Kinetics of charge transfer in DNA containing a mismatch
title_full_unstemmed Kinetics of charge transfer in DNA containing a mismatch
title_short Kinetics of charge transfer in DNA containing a mismatch
title_sort kinetics of charge transfer in dna containing a mismatch
topic Chemistry and Synthetic Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553589/
https://www.ncbi.nlm.nih.gov/pubmed/18757889
http://dx.doi.org/10.1093/nar/gkn505
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AT majimatetsuro kineticsofchargetransferindnacontainingamismatch