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Kinetics of charge transfer in DNA containing a mismatch
Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553589/ https://www.ncbi.nlm.nih.gov/pubmed/18757889 http://dx.doi.org/10.1093/nar/gkn505 |
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author | Osakada, Yasuko Kawai, Kiyohiko Fujitsuka, Mamoru Majima, Tetsuro |
author_facet | Osakada, Yasuko Kawai, Kiyohiko Fujitsuka, Mamoru Majima, Tetsuro |
author_sort | Osakada, Yasuko |
collection | PubMed |
description | Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch. |
format | Text |
id | pubmed-2553589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25535892008-10-01 Kinetics of charge transfer in DNA containing a mismatch Osakada, Yasuko Kawai, Kiyohiko Fujitsuka, Mamoru Majima, Tetsuro Nucleic Acids Res Chemistry and Synthetic Biology Charge transfer (CT) in DNA offers a unique approach for the detection of a single-base mismatch in a DNA molecule. While the single-base mismatch would significantly affect the CT in DNA, the kinetic basis for the drastic decrease in the CT efficiency through DNA containing mismatches still remains unclear. Recently, we determined the rate constants of the CT through the fully matched DNA, and we can now estimate the CT rate constant for a certain fully matched sequence. We assumed that further elucidating of the kinetics in mismatched sequences can lead to the discrimination of the DNA single-base mismatch based on the kinetics. In this study, we investigated the detailed kinetics of the CT through DNA containing mismatches and tried to discriminate a mismatch sequence based on the kinetics of the CT in DNA containing a mismatch. Oxford University Press 2008-10 2008-08-30 /pmc/articles/PMC2553589/ /pubmed/18757889 http://dx.doi.org/10.1093/nar/gkn505 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Chemistry and Synthetic Biology Osakada, Yasuko Kawai, Kiyohiko Fujitsuka, Mamoru Majima, Tetsuro Kinetics of charge transfer in DNA containing a mismatch |
title | Kinetics of charge transfer in DNA containing a mismatch |
title_full | Kinetics of charge transfer in DNA containing a mismatch |
title_fullStr | Kinetics of charge transfer in DNA containing a mismatch |
title_full_unstemmed | Kinetics of charge transfer in DNA containing a mismatch |
title_short | Kinetics of charge transfer in DNA containing a mismatch |
title_sort | kinetics of charge transfer in dna containing a mismatch |
topic | Chemistry and Synthetic Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553589/ https://www.ncbi.nlm.nih.gov/pubmed/18757889 http://dx.doi.org/10.1093/nar/gkn505 |
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