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LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens
BACKGROUND: Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a s...
| Autores principales: | , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2553803/ https://www.ncbi.nlm.nih.gov/pubmed/18783594 http://dx.doi.org/10.1186/1471-2105-9-368 |