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Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study

The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determinin...

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Detalles Bibliográficos
Autores principales: Walker, Azida, Udupa, Kodetthoor B, Chowdhury, Parimal
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556029/
https://www.ncbi.nlm.nih.gov/pubmed/18822160
http://dx.doi.org/10.1186/1617-9625-4-5
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author Walker, Azida
Udupa, Kodetthoor B
Chowdhury, Parimal
author_facet Walker, Azida
Udupa, Kodetthoor B
Chowdhury, Parimal
author_sort Walker, Azida
collection PubMed
description The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determining cell proliferation through the activation of ERK signaling, basal and CCK-stimulated cell function and measuring lipid peroxidation. AR42J cells have been exposed to either a non-cytotoxic dose of 20 μM H(2)O(2 )for 15 min or to 100 μM of nicotine for 3 min respectively. Nicotine and H(2)O(2 )at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Immunofluorescence studies employing specific antibody to p-ERK1/2 confirmed the latter. Nicotine-induced increase in the proliferation of AR42J cells was significantly higher in comparison to H(2)O(2 )exposed cells. CCK-stimulated cell function induced by nicotine was significantly higher in AR42J cells as compared to the response by H(2)O(2). These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. The data suggests that nicotine-induced mitogenic response in AR42J cells closely identifies the response induced by an oxidative biomarker.
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spelling pubmed-25560292008-09-29 Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study Walker, Azida Udupa, Kodetthoor B Chowdhury, Parimal Tob Induc Dis Research The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determining cell proliferation through the activation of ERK signaling, basal and CCK-stimulated cell function and measuring lipid peroxidation. AR42J cells have been exposed to either a non-cytotoxic dose of 20 μM H(2)O(2 )for 15 min or to 100 μM of nicotine for 3 min respectively. Nicotine and H(2)O(2 )at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Immunofluorescence studies employing specific antibody to p-ERK1/2 confirmed the latter. Nicotine-induced increase in the proliferation of AR42J cells was significantly higher in comparison to H(2)O(2 )exposed cells. CCK-stimulated cell function induced by nicotine was significantly higher in AR42J cells as compared to the response by H(2)O(2). These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. The data suggests that nicotine-induced mitogenic response in AR42J cells closely identifies the response induced by an oxidative biomarker. BioMed Central 2008-07-31 /pmc/articles/PMC2556029/ /pubmed/18822160 http://dx.doi.org/10.1186/1617-9625-4-5 Text en Copyright © 2008 Walker et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Walker, Azida
Udupa, Kodetthoor B
Chowdhury, Parimal
Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title_full Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title_fullStr Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title_full_unstemmed Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title_short Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
title_sort mitogenic and functional responses by nicotine and hydrogen peroxide in ar42j cells: a comparative study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556029/
https://www.ncbi.nlm.nih.gov/pubmed/18822160
http://dx.doi.org/10.1186/1617-9625-4-5
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