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Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determinin...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556029/ https://www.ncbi.nlm.nih.gov/pubmed/18822160 http://dx.doi.org/10.1186/1617-9625-4-5 |
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author | Walker, Azida Udupa, Kodetthoor B Chowdhury, Parimal |
author_facet | Walker, Azida Udupa, Kodetthoor B Chowdhury, Parimal |
author_sort | Walker, Azida |
collection | PubMed |
description | The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determining cell proliferation through the activation of ERK signaling, basal and CCK-stimulated cell function and measuring lipid peroxidation. AR42J cells have been exposed to either a non-cytotoxic dose of 20 μM H(2)O(2 )for 15 min or to 100 μM of nicotine for 3 min respectively. Nicotine and H(2)O(2 )at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Immunofluorescence studies employing specific antibody to p-ERK1/2 confirmed the latter. Nicotine-induced increase in the proliferation of AR42J cells was significantly higher in comparison to H(2)O(2 )exposed cells. CCK-stimulated cell function induced by nicotine was significantly higher in AR42J cells as compared to the response by H(2)O(2). These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. The data suggests that nicotine-induced mitogenic response in AR42J cells closely identifies the response induced by an oxidative biomarker. |
format | Text |
id | pubmed-2556029 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-25560292008-09-29 Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study Walker, Azida Udupa, Kodetthoor B Chowdhury, Parimal Tob Induc Dis Research The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H(2)O(2)) was used as a source of known oxidative biomarker. Responses were examined by determining cell proliferation through the activation of ERK signaling, basal and CCK-stimulated cell function and measuring lipid peroxidation. AR42J cells have been exposed to either a non-cytotoxic dose of 20 μM H(2)O(2 )for 15 min or to 100 μM of nicotine for 3 min respectively. Nicotine and H(2)O(2 )at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Immunofluorescence studies employing specific antibody to p-ERK1/2 confirmed the latter. Nicotine-induced increase in the proliferation of AR42J cells was significantly higher in comparison to H(2)O(2 )exposed cells. CCK-stimulated cell function induced by nicotine was significantly higher in AR42J cells as compared to the response by H(2)O(2). These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. The data suggests that nicotine-induced mitogenic response in AR42J cells closely identifies the response induced by an oxidative biomarker. BioMed Central 2008-07-31 /pmc/articles/PMC2556029/ /pubmed/18822160 http://dx.doi.org/10.1186/1617-9625-4-5 Text en Copyright © 2008 Walker et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Walker, Azida Udupa, Kodetthoor B Chowdhury, Parimal Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title | Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title_full | Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title_fullStr | Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title_full_unstemmed | Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title_short | Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study |
title_sort | mitogenic and functional responses by nicotine and hydrogen peroxide in ar42j cells: a comparative study |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556029/ https://www.ncbi.nlm.nih.gov/pubmed/18822160 http://dx.doi.org/10.1186/1617-9625-4-5 |
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