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The keratin-binding protein Albatross regulates polarization of epithelial cells
The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane id...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557036/ https://www.ncbi.nlm.nih.gov/pubmed/18838552 http://dx.doi.org/10.1083/jcb.200803133 |
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author | Sugimoto, Masahiko Inoko, Akihito Shiromizu, Takashi Nakayama, Masanori Zou, Peng Yonemura, Shigenobu Hayashi, Yuko Izawa, Ichiro Sasoh, Mikio Uji, Yukitaka Kaibuchi, Kozo Kiyono, Tohru Inagaki, Masaki |
author_facet | Sugimoto, Masahiko Inoko, Akihito Shiromizu, Takashi Nakayama, Masanori Zou, Peng Yonemura, Shigenobu Hayashi, Yuko Izawa, Ichiro Sasoh, Mikio Uji, Yukitaka Kaibuchi, Kozo Kiyono, Tohru Inagaki, Masaki |
author_sort | Sugimoto, Masahiko |
collection | PubMed |
description | The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane identity. In nonpolarized epithelial cells, Albatross localizes with keratin filaments, whereas in polarized epithelial cells, Albatross is primarily localized in the vicinity of the AJC. Knockdown of Albatross in polarized cells causes a disappearance of key components of the AJC at cell–cell borders and keratin filament reorganization. Lateral proteins E-cadherin and desmoglein 2 were mislocalized even on the apical side. Although Albatross promotes localization of Par3 to the AJC, Par3 and ezrin are still retained at the apical surface in Albatross knockdown cells, which retain intact microvilli. Analysis of keratin-deficient epithelial cells revealed that keratins are required to stabilize the Albatross protein, thus promoting the formation of AJC. We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization. |
format | Text |
id | pubmed-2557036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25570362009-04-06 The keratin-binding protein Albatross regulates polarization of epithelial cells Sugimoto, Masahiko Inoko, Akihito Shiromizu, Takashi Nakayama, Masanori Zou, Peng Yonemura, Shigenobu Hayashi, Yuko Izawa, Ichiro Sasoh, Mikio Uji, Yukitaka Kaibuchi, Kozo Kiyono, Tohru Inagaki, Masaki J Cell Biol Research Articles The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane identity. In nonpolarized epithelial cells, Albatross localizes with keratin filaments, whereas in polarized epithelial cells, Albatross is primarily localized in the vicinity of the AJC. Knockdown of Albatross in polarized cells causes a disappearance of key components of the AJC at cell–cell borders and keratin filament reorganization. Lateral proteins E-cadherin and desmoglein 2 were mislocalized even on the apical side. Although Albatross promotes localization of Par3 to the AJC, Par3 and ezrin are still retained at the apical surface in Albatross knockdown cells, which retain intact microvilli. Analysis of keratin-deficient epithelial cells revealed that keratins are required to stabilize the Albatross protein, thus promoting the formation of AJC. We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557036/ /pubmed/18838552 http://dx.doi.org/10.1083/jcb.200803133 Text en © 2008 Sugimoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Sugimoto, Masahiko Inoko, Akihito Shiromizu, Takashi Nakayama, Masanori Zou, Peng Yonemura, Shigenobu Hayashi, Yuko Izawa, Ichiro Sasoh, Mikio Uji, Yukitaka Kaibuchi, Kozo Kiyono, Tohru Inagaki, Masaki The keratin-binding protein Albatross regulates polarization of epithelial cells |
title | The keratin-binding protein Albatross regulates polarization of epithelial cells |
title_full | The keratin-binding protein Albatross regulates polarization of epithelial cells |
title_fullStr | The keratin-binding protein Albatross regulates polarization of epithelial cells |
title_full_unstemmed | The keratin-binding protein Albatross regulates polarization of epithelial cells |
title_short | The keratin-binding protein Albatross regulates polarization of epithelial cells |
title_sort | keratin-binding protein albatross regulates polarization of epithelial cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557036/ https://www.ncbi.nlm.nih.gov/pubmed/18838552 http://dx.doi.org/10.1083/jcb.200803133 |
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