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The keratin-binding protein Albatross regulates polarization of epithelial cells

The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane id...

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Autores principales: Sugimoto, Masahiko, Inoko, Akihito, Shiromizu, Takashi, Nakayama, Masanori, Zou, Peng, Yonemura, Shigenobu, Hayashi, Yuko, Izawa, Ichiro, Sasoh, Mikio, Uji, Yukitaka, Kaibuchi, Kozo, Kiyono, Tohru, Inagaki, Masaki
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557036/
https://www.ncbi.nlm.nih.gov/pubmed/18838552
http://dx.doi.org/10.1083/jcb.200803133
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author Sugimoto, Masahiko
Inoko, Akihito
Shiromizu, Takashi
Nakayama, Masanori
Zou, Peng
Yonemura, Shigenobu
Hayashi, Yuko
Izawa, Ichiro
Sasoh, Mikio
Uji, Yukitaka
Kaibuchi, Kozo
Kiyono, Tohru
Inagaki, Masaki
author_facet Sugimoto, Masahiko
Inoko, Akihito
Shiromizu, Takashi
Nakayama, Masanori
Zou, Peng
Yonemura, Shigenobu
Hayashi, Yuko
Izawa, Ichiro
Sasoh, Mikio
Uji, Yukitaka
Kaibuchi, Kozo
Kiyono, Tohru
Inagaki, Masaki
author_sort Sugimoto, Masahiko
collection PubMed
description The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane identity. In nonpolarized epithelial cells, Albatross localizes with keratin filaments, whereas in polarized epithelial cells, Albatross is primarily localized in the vicinity of the AJC. Knockdown of Albatross in polarized cells causes a disappearance of key components of the AJC at cell–cell borders and keratin filament reorganization. Lateral proteins E-cadherin and desmoglein 2 were mislocalized even on the apical side. Although Albatross promotes localization of Par3 to the AJC, Par3 and ezrin are still retained at the apical surface in Albatross knockdown cells, which retain intact microvilli. Analysis of keratin-deficient epithelial cells revealed that keratins are required to stabilize the Albatross protein, thus promoting the formation of AJC. We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization.
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spelling pubmed-25570362009-04-06 The keratin-binding protein Albatross regulates polarization of epithelial cells Sugimoto, Masahiko Inoko, Akihito Shiromizu, Takashi Nakayama, Masanori Zou, Peng Yonemura, Shigenobu Hayashi, Yuko Izawa, Ichiro Sasoh, Mikio Uji, Yukitaka Kaibuchi, Kozo Kiyono, Tohru Inagaki, Masaki J Cell Biol Research Articles The keratin intermediate filament network is abundant in epithelial cells, but its function in the establishment and maintenance of cell polarity is unclear. Here, we show that Albatross complexes with Par3 to regulate formation of the apical junctional complex (AJC) and maintain lateral membrane identity. In nonpolarized epithelial cells, Albatross localizes with keratin filaments, whereas in polarized epithelial cells, Albatross is primarily localized in the vicinity of the AJC. Knockdown of Albatross in polarized cells causes a disappearance of key components of the AJC at cell–cell borders and keratin filament reorganization. Lateral proteins E-cadherin and desmoglein 2 were mislocalized even on the apical side. Although Albatross promotes localization of Par3 to the AJC, Par3 and ezrin are still retained at the apical surface in Albatross knockdown cells, which retain intact microvilli. Analysis of keratin-deficient epithelial cells revealed that keratins are required to stabilize the Albatross protein, thus promoting the formation of AJC. We propose that keratins and the keratin-binding protein Albatross are important for epithelial cell polarization. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557036/ /pubmed/18838552 http://dx.doi.org/10.1083/jcb.200803133 Text en © 2008 Sugimoto et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Sugimoto, Masahiko
Inoko, Akihito
Shiromizu, Takashi
Nakayama, Masanori
Zou, Peng
Yonemura, Shigenobu
Hayashi, Yuko
Izawa, Ichiro
Sasoh, Mikio
Uji, Yukitaka
Kaibuchi, Kozo
Kiyono, Tohru
Inagaki, Masaki
The keratin-binding protein Albatross regulates polarization of epithelial cells
title The keratin-binding protein Albatross regulates polarization of epithelial cells
title_full The keratin-binding protein Albatross regulates polarization of epithelial cells
title_fullStr The keratin-binding protein Albatross regulates polarization of epithelial cells
title_full_unstemmed The keratin-binding protein Albatross regulates polarization of epithelial cells
title_short The keratin-binding protein Albatross regulates polarization of epithelial cells
title_sort keratin-binding protein albatross regulates polarization of epithelial cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557036/
https://www.ncbi.nlm.nih.gov/pubmed/18838552
http://dx.doi.org/10.1083/jcb.200803133
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