Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination

Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte cult...

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Detalles Bibliográficos
Autores principales: Xi, Zhiyong, Das, Suchismita, Garver, Lindsey, Dimopoulos, George
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2007
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557090/
https://www.ncbi.nlm.nih.gov/pubmed/18979020
http://dx.doi.org/10.3791/222
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author Xi, Zhiyong
Das, Suchismita
Garver, Lindsey
Dimopoulos, George
author_facet Xi, Zhiyong
Das, Suchismita
Garver, Lindsey
Dimopoulos, George
author_sort Xi, Zhiyong
collection PubMed
description Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated.
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spelling pubmed-25570902008-10-31 Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination Xi, Zhiyong Das, Suchismita Garver, Lindsey Dimopoulos, George J Vis Exp Cellular Biology Once a gene is identified as potentially refractory for malaria, it must be evaluated for its role in preventing Plasmodium infections within the mosquito. This protocol illustrates how the extent of plasmodium infections of mosquitoes can be assayed. The techniques for preparing the gametocyte culture, membrane feeding mosquitoes human blood, and assaying viral titers in the mosquito midgut are demonstrated. MyJove Corporation 2007-07-04 /pmc/articles/PMC2557090/ /pubmed/18979020 http://dx.doi.org/10.3791/222 Text en Copyright © 2007, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Cellular Biology
Xi, Zhiyong
Das, Suchismita
Garver, Lindsey
Dimopoulos, George
Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title_full Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title_fullStr Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title_full_unstemmed Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title_short Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
title_sort protocol for plasmodium falciparum infections in mosquitoes and infection phenotype determination
topic Cellular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557090/
https://www.ncbi.nlm.nih.gov/pubmed/18979020
http://dx.doi.org/10.3791/222
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AT garverlindsey protocolforplasmodiumfalciparuminfectionsinmosquitoesandinfectionphenotypedetermination
AT dimopoulosgeorge protocolforplasmodiumfalciparuminfectionsinmosquitoesandinfectionphenotypedetermination

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