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Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana

BACKGROUND: Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated...

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Autores principales: Krishnaswamy, Sowmya S, Srivastava, Sanjeeva, Mohammadi, Mohsen, Rahman, Muhammad H, Deyholos, Michael K, Kav, Nat NV
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2559843/
https://www.ncbi.nlm.nih.gov/pubmed/18783601
http://dx.doi.org/10.1186/1471-2229-8-91
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author Krishnaswamy, Sowmya S
Srivastava, Sanjeeva
Mohammadi, Mohsen
Rahman, Muhammad H
Deyholos, Michael K
Kav, Nat NV
author_facet Krishnaswamy, Sowmya S
Srivastava, Sanjeeva
Mohammadi, Mohsen
Rahman, Muhammad H
Deyholos, Michael K
Kav, Nat NV
author_sort Krishnaswamy, Sowmya S
collection PubMed
description BACKGROUND: Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated that the constitutive expression of pea ABR17 cDNA in Arabidopsis thaliana and Brassica napus enhances their germination and early seedling growth under stress. Although it has been reported that several members of the PR10 family including ABR17 possess RNase activity, the exact mechanism by which the aforementioned characteristics are conferred by ABR17 is unknown at this time. We hypothesized that a study of differences in transcriptome between wild type (WT) and ABR17 transgenic A. thaliana may shed light on this process. RESULTS: The molecular changes brought about by the expression of pea ABR17 cDNA in A. thaliana in the presence or absence of salt stress were investigated using microarrays consisting of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes. Statistical analysis identified number of genes which were over represented among up- or down-regulated transcripts in the transgenic line. Our results highlight the important roles of many abscisic acid (ABA) and cytokinin (CK) responsive genes in ABR17 transgenic lines. Although the transcriptional changes followed a general salt response theme in both WT and transgenic seedlings under salt stress, many genes exhibited differential expression patterns when the transgenic and WT lines were compared. These genes include plant defensins, heat shock proteins, other defense related genes, and several transcriptional factors. Our microarray results for selected genes were validated using quantitative real-time PCR. CONCLUSION: Transcriptional analysis in ABR17 transgenic Arabidopsis plants, both under normal and saline conditions, revealed significant changes in abundance of transcripts for many stress responsive genes, as well as those related to plant growth and development. Our results also suggest that ABR17 may mediate stress tolerance through the modulation of many ABA- and CK-responsive genes and may further our understanding of the role of ABR17 in mediating plant stress responses.
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spelling pubmed-25598432008-10-03 Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana Krishnaswamy, Sowmya S Srivastava, Sanjeeva Mohammadi, Mohsen Rahman, Muhammad H Deyholos, Michael K Kav, Nat NV BMC Plant Biol Research Article BACKGROUND: Pathogenesis-related proteins belonging to group 10 (PR10) are elevated in response to biotic and abiotic stresses in plants. Previously, we have shown a drastic salinity-induced increase in the levels of ABR17, a member of the PR10 family, in pea. Furthermore, we have also demonstrated that the constitutive expression of pea ABR17 cDNA in Arabidopsis thaliana and Brassica napus enhances their germination and early seedling growth under stress. Although it has been reported that several members of the PR10 family including ABR17 possess RNase activity, the exact mechanism by which the aforementioned characteristics are conferred by ABR17 is unknown at this time. We hypothesized that a study of differences in transcriptome between wild type (WT) and ABR17 transgenic A. thaliana may shed light on this process. RESULTS: The molecular changes brought about by the expression of pea ABR17 cDNA in A. thaliana in the presence or absence of salt stress were investigated using microarrays consisting of 70-mer oligonucleotide probes representing 23,686 Arabidopsis genes. Statistical analysis identified number of genes which were over represented among up- or down-regulated transcripts in the transgenic line. Our results highlight the important roles of many abscisic acid (ABA) and cytokinin (CK) responsive genes in ABR17 transgenic lines. Although the transcriptional changes followed a general salt response theme in both WT and transgenic seedlings under salt stress, many genes exhibited differential expression patterns when the transgenic and WT lines were compared. These genes include plant defensins, heat shock proteins, other defense related genes, and several transcriptional factors. Our microarray results for selected genes were validated using quantitative real-time PCR. CONCLUSION: Transcriptional analysis in ABR17 transgenic Arabidopsis plants, both under normal and saline conditions, revealed significant changes in abundance of transcripts for many stress responsive genes, as well as those related to plant growth and development. Our results also suggest that ABR17 may mediate stress tolerance through the modulation of many ABA- and CK-responsive genes and may further our understanding of the role of ABR17 in mediating plant stress responses. BioMed Central 2008-09-10 /pmc/articles/PMC2559843/ /pubmed/18783601 http://dx.doi.org/10.1186/1471-2229-8-91 Text en Copyright © 2008 Krishnaswamy et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Krishnaswamy, Sowmya S
Srivastava, Sanjeeva
Mohammadi, Mohsen
Rahman, Muhammad H
Deyholos, Michael K
Kav, Nat NV
Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title_full Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title_fullStr Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title_full_unstemmed Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title_short Transcriptional profiling of pea ABR17 mediated changes in gene expression in Arabidopsis thaliana
title_sort transcriptional profiling of pea abr17 mediated changes in gene expression in arabidopsis thaliana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2559843/
https://www.ncbi.nlm.nih.gov/pubmed/18783601
http://dx.doi.org/10.1186/1471-2229-8-91
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