Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin

BACKGROUND: Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-ta...

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Autores principales: Cai, Xiu-Mei, Xie, Hai-Long, Liu, Ming-Zhu, Zha, Xi-Liang
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562374/
https://www.ncbi.nlm.nih.gov/pubmed/18801171
http://dx.doi.org/10.1186/1472-6750-8-74
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author Cai, Xiu-Mei
Xie, Hai-Long
Liu, Ming-Zhu
Zha, Xi-Liang
author_facet Cai, Xiu-Mei
Xie, Hai-Long
Liu, Ming-Zhu
Zha, Xi-Liang
author_sort Cai, Xiu-Mei
collection PubMed
description BACKGROUND: Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-targeted phagemid particles carrying siRNA would be a promising tool for anti-cancer siRNA delivery. RESULTS: We found that pSi4.1-siFAK phagemid particles could significantly inhibit the expression of focal adhesion kinase in the HCPT-treated cells. Moreover, we also observed that the particles could potently suppress cell growth and cell invasion. CONCLUSION: These results indicated that EGF-targeted phagemid particles might be a promising tool for anti-cancer siRNA delivery in the presence of HCPT.
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spelling pubmed-25623742008-10-07 Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin Cai, Xiu-Mei Xie, Hai-Long Liu, Ming-Zhu Zha, Xi-Liang BMC Biotechnol Methodology Article BACKGROUND: Previous studies demonstrated the EGF-targeted phagemid particles carrying siRNA against Akt could be expressed efficiently in the presence of hydroxycamptothecin (HCPT). However, no significant cell growth inhibition was obtained. This study was to further investigate whether the EGF-targeted phagemid particles carrying siRNA would be a promising tool for anti-cancer siRNA delivery. RESULTS: We found that pSi4.1-siFAK phagemid particles could significantly inhibit the expression of focal adhesion kinase in the HCPT-treated cells. Moreover, we also observed that the particles could potently suppress cell growth and cell invasion. CONCLUSION: These results indicated that EGF-targeted phagemid particles might be a promising tool for anti-cancer siRNA delivery in the presence of HCPT. BioMed Central 2008-09-18 /pmc/articles/PMC2562374/ /pubmed/18801171 http://dx.doi.org/10.1186/1472-6750-8-74 Text en Copyright © 2008 Cai et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Article
Cai, Xiu-Mei
Xie, Hai-Long
Liu, Ming-Zhu
Zha, Xi-Liang
Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title_full Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title_fullStr Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title_full_unstemmed Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title_short Inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying siRNA against focal adhesion kinase in the presence of hydroxycamptothecin
title_sort inhibition of cell growth and invasion by epidermal growth factor-targeted phagemid particles carrying sirna against focal adhesion kinase in the presence of hydroxycamptothecin
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562374/
https://www.ncbi.nlm.nih.gov/pubmed/18801171
http://dx.doi.org/10.1186/1472-6750-8-74
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