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A fully automated liquid–liquid extraction system utilizing interface detection
The development of the Abbott Liquid-Liquid Extraction Station was a result of the need for an automated system to perform aqueous extraction on large sets of newly synthesized organic compounds used for drug discovery. The system utilizes a cylindrical laboratory robot to shuttle sample vials betwe...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562833/ https://www.ncbi.nlm.nih.gov/pubmed/18924693 http://dx.doi.org/10.1155/S146392460000033X |
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author | Maslana, Eugene Schmitt, Robert Pan, Jeffrey |
author_facet | Maslana, Eugene Schmitt, Robert Pan, Jeffrey |
author_sort | Maslana, Eugene |
collection | PubMed |
description | The development of the Abbott Liquid-Liquid Extraction Station was a result of the need for an automated system to perform aqueous extraction on large sets of newly synthesized organic compounds used for drug discovery. The system utilizes a cylindrical laboratory robot to shuttle sample vials between two loading racks, two identical extraction stations, and a centrifuge. Extraction is performed by detecting the phase interface (by difference in refractive index) of the moving column of fluid drawn from the bottom of each vial containing a biphasic mixture. The integration of interface detection with fluid extraction maximizes sample throughput. Abbott-developed electronics process the detector signals. Sample mixing is performed by high-speed solvent injection. Centrifuging of the samples reduces interface emulsions. Operating software permits the user to program wash protocols with any one of six solvents per wash cycle with as many cycle repeats as necessary. Station capacity is eighty, 15 ml vials. This system has proven successful with a broad spectrum of both ethyl acetate and methylene chloride based chemistries. The development and characterization of this automated extraction system will be presented. |
format | Text |
id | pubmed-2562833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-25628332008-10-16 A fully automated liquid–liquid extraction system utilizing interface detection Maslana, Eugene Schmitt, Robert Pan, Jeffrey J Autom Methods Manag Chem Research Article The development of the Abbott Liquid-Liquid Extraction Station was a result of the need for an automated system to perform aqueous extraction on large sets of newly synthesized organic compounds used for drug discovery. The system utilizes a cylindrical laboratory robot to shuttle sample vials between two loading racks, two identical extraction stations, and a centrifuge. Extraction is performed by detecting the phase interface (by difference in refractive index) of the moving column of fluid drawn from the bottom of each vial containing a biphasic mixture. The integration of interface detection with fluid extraction maximizes sample throughput. Abbott-developed electronics process the detector signals. Sample mixing is performed by high-speed solvent injection. Centrifuging of the samples reduces interface emulsions. Operating software permits the user to program wash protocols with any one of six solvents per wash cycle with as many cycle repeats as necessary. Station capacity is eighty, 15 ml vials. This system has proven successful with a broad spectrum of both ethyl acetate and methylene chloride based chemistries. The development and characterization of this automated extraction system will be presented. Hindawi Publishing Corporation 2000 /pmc/articles/PMC2562833/ /pubmed/18924693 http://dx.doi.org/10.1155/S146392460000033X Text en Copyright © 2000 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Maslana, Eugene Schmitt, Robert Pan, Jeffrey A fully automated liquid–liquid extraction system utilizing interface detection |
title | A fully automated liquid–liquid extraction system utilizing interface detection |
title_full | A fully automated liquid–liquid extraction system utilizing interface detection |
title_fullStr | A fully automated liquid–liquid extraction system utilizing interface detection |
title_full_unstemmed | A fully automated liquid–liquid extraction system utilizing interface detection |
title_short | A fully automated liquid–liquid extraction system utilizing interface detection |
title_sort | fully automated liquid–liquid extraction system utilizing interface detection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562833/ https://www.ncbi.nlm.nih.gov/pubmed/18924693 http://dx.doi.org/10.1155/S146392460000033X |
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