Speciation of inorganic selenium and selenoamino acids by an HPLC-UV-HG-AFS system

For the on-line speciation of selenocystine (SeCys), selenomethionine (SeMet), selenoethionine (SeEt), selenite (Se(IV)) and selenate (Se(VI)), a high-performance liquid chromatography-UV irradiation-hydride generation-atomic fluorescence spectro- metric method is described. Separation was carried o...

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Detalles Bibliográficos
Autores principales: Ipolyi, I., Corns, W., Stockwell, P., Fodor, P.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2001
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2562854/
https://www.ncbi.nlm.nih.gov/pubmed/18924707
http://dx.doi.org/10.1155/S1463924601000207
Descripción
Sumario:For the on-line speciation of selenocystine (SeCys), selenomethionine (SeMet), selenoethionine (SeEt), selenite (Se(IV)) and selenate (Se(VI)), a high-performance liquid chromatography-UV irradiation-hydride generation-atomic fluorescence spectro- metric method is described. Separation was carried out on a conventional reversed-phase C18 column modified with didodecyl- dimethylammonium bromide with gradient elution applying two concentrations of ammonium acetate as the mobile phase. UV irradiation and hydride generation parameters were optimized. The obtained detection limits for SeCys, SeMet, SeEt, Se(IV) and Se(VI) were 0.31, 0.43, 0.7, 0.44 and 0.32 ng ml(−)1, respectively, using a 100-wl loop. The method was tested with spiked mineral water and two volunteers' urine samples.

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