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Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage

BACKGROUND: White adipose tissue is not only an energy storage organ; it also functions as an endocrine organ. The coordination and integration of numerous gene expression events is required to establish and maintain the adipocyte phenotype. FINDINGS: We previously observed a 45-fold upregulation fo...

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Autores principales: Wu, Yu, Smas, Cynthia M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2564950/
https://www.ncbi.nlm.nih.gov/pubmed/18803820
http://dx.doi.org/10.1186/1756-0500-1-85
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author Wu, Yu
Smas, Cynthia M
author_facet Wu, Yu
Smas, Cynthia M
author_sort Wu, Yu
collection PubMed
description BACKGROUND: White adipose tissue is not only an energy storage organ; it also functions as an endocrine organ. The coordination and integration of numerous gene expression events is required to establish and maintain the adipocyte phenotype. FINDINGS: We previously observed a 45-fold upregulation for a transcript encoding a novel predicted transmembrane protein, Tmem182, upon brown preadipocyte to adipocyte conversion. Here we use real-time PCR analysis to further characterize Tmem182 transcript expression in the adipocyte lineage. Analysis across a panel of 10 murine tissues revealed highest Tmem182 transcript expression in white adipose tissues (WAT), with 10-fold to 20-fold higher levels than in brown adipose tissue (BAT). Tmem182 transcript expression is ~3-fold upregulated in BAT of genetically obese (ob/ob) mice vs. wild type C57BL/6. Analysis of three in vitro models of white adipogenesis indicates markedly enriched expression of Tmem182 transcript in adipocytes vs. preadipocytes. Compared to 3T3-L1 preadipocytes, a 157-fold higher level of Tmem182 transcript is detected at 3 day post-induction of adipogenesis and an ~2500-fold higher level in mature 3T3-L1 adipocytes. TNFα treatment of 3T3-L1 adipocytes resulted in a ~90% decrease in Tmem182 transcript level. As skeletal muscle and heart were also found to express Tmem182 transcript, we assessed expression in C2C12 myogenesis and observed a ~770-fold upregulation upon conversion of myoblasts to myocytes. CONCLUSION: WAT is the most prominent site of Tmem182 transcript expression and levels of transcript for Tmem182 are altered in adipose tissues of ob/ob mice and upon exposure of 3T3-L1 adipocytes to the proinflammatory cytokine TNFα. The dramatic upregulation of Tmem182 transcript during in vitro adipogenesis and myogenesis suggests Tmem182 may function in intracellular pathways important in these two cell types.
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spelling pubmed-25649502008-10-09 Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage Wu, Yu Smas, Cynthia M BMC Res Notes Short Report BACKGROUND: White adipose tissue is not only an energy storage organ; it also functions as an endocrine organ. The coordination and integration of numerous gene expression events is required to establish and maintain the adipocyte phenotype. FINDINGS: We previously observed a 45-fold upregulation for a transcript encoding a novel predicted transmembrane protein, Tmem182, upon brown preadipocyte to adipocyte conversion. Here we use real-time PCR analysis to further characterize Tmem182 transcript expression in the adipocyte lineage. Analysis across a panel of 10 murine tissues revealed highest Tmem182 transcript expression in white adipose tissues (WAT), with 10-fold to 20-fold higher levels than in brown adipose tissue (BAT). Tmem182 transcript expression is ~3-fold upregulated in BAT of genetically obese (ob/ob) mice vs. wild type C57BL/6. Analysis of three in vitro models of white adipogenesis indicates markedly enriched expression of Tmem182 transcript in adipocytes vs. preadipocytes. Compared to 3T3-L1 preadipocytes, a 157-fold higher level of Tmem182 transcript is detected at 3 day post-induction of adipogenesis and an ~2500-fold higher level in mature 3T3-L1 adipocytes. TNFα treatment of 3T3-L1 adipocytes resulted in a ~90% decrease in Tmem182 transcript level. As skeletal muscle and heart were also found to express Tmem182 transcript, we assessed expression in C2C12 myogenesis and observed a ~770-fold upregulation upon conversion of myoblasts to myocytes. CONCLUSION: WAT is the most prominent site of Tmem182 transcript expression and levels of transcript for Tmem182 are altered in adipose tissues of ob/ob mice and upon exposure of 3T3-L1 adipocytes to the proinflammatory cytokine TNFα. The dramatic upregulation of Tmem182 transcript during in vitro adipogenesis and myogenesis suggests Tmem182 may function in intracellular pathways important in these two cell types. BioMed Central 2008-09-19 /pmc/articles/PMC2564950/ /pubmed/18803820 http://dx.doi.org/10.1186/1756-0500-1-85 Text en Copyright © 2008 Wu and Smas; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Wu, Yu
Smas, Cynthia M
Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title_full Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title_fullStr Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title_full_unstemmed Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title_short Expression and regulation of transcript for the novel transmembrane protein Tmem182 in the adipocyte and muscle lineage
title_sort expression and regulation of transcript for the novel transmembrane protein tmem182 in the adipocyte and muscle lineage
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2564950/
https://www.ncbi.nlm.nih.gov/pubmed/18803820
http://dx.doi.org/10.1186/1756-0500-1-85
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