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Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus

The effects of donor age and site of placement on the survival of fetal medial habenula (MH) transplants into adult rats hosts were examined. The innervation of the interpeduncular nucleus (IPN) in such cases was also examined. Explants of MH consisting of the medial-dorsal lip of the third ventricl...

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Detalles Bibliográficos
Autores principales: Lenn, Nicholas J., Seeley, P. John, Field, Pauline M., Raisman, Geoffrey
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565001/
https://www.ncbi.nlm.nih.gov/pubmed/2485117
http://dx.doi.org/10.1155/NP.1989.57
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author Lenn, Nicholas J.
Seeley, P. John
Field, Pauline M.
Raisman, Geoffrey
author_facet Lenn, Nicholas J.
Seeley, P. John
Field, Pauline M.
Raisman, Geoffrey
author_sort Lenn, Nicholas J.
collection PubMed
description The effects of donor age and site of placement on the survival of fetal medial habenula (MH) transplants into adult rats hosts were examined. The innervation of the interpeduncular nucleus (IPN) in such cases was also examined. Explants of MH consisting of the medial-dorsal lip of the third ventricle were held in vitro for 1—2 days. Colloidal gold conjugated to wheat germ agglutinin was added for the last 18 hours to label the cells. Four of 16 cases with E19 derived transplants contained donor neurons. Markedly larger transplants were present in 95% of 20 cases with E16 derived transplants. Sites in the ventral midbrain were successful, while limited or no survival occurred at sites more remote from IPN. Retrograde labeling of transplant neurons was present in each case studied with HRP injection into host IPN. Colloidal gold-labeled macrophages, some oriented capillaries and GFAP-positive processes marked the donor-host interface. In EM the interface was evident only by the difference in tissue elements in the transplant versus host. Numerous synapses of Gray types I and II were present in the transplant. Excellent survival of MH neurons, donor/host interfaces, innervation of IPN by the transplant and fine structure in and around the transplants, all suggest that such preparations are suitable for further experimental analysis of the habenulo-interpeduncular system.
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spelling pubmed-25650012008-10-16 Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus Lenn, Nicholas J. Seeley, P. John Field, Pauline M. Raisman, Geoffrey J Neural Transplant Article The effects of donor age and site of placement on the survival of fetal medial habenula (MH) transplants into adult rats hosts were examined. The innervation of the interpeduncular nucleus (IPN) in such cases was also examined. Explants of MH consisting of the medial-dorsal lip of the third ventricle were held in vitro for 1—2 days. Colloidal gold conjugated to wheat germ agglutinin was added for the last 18 hours to label the cells. Four of 16 cases with E19 derived transplants contained donor neurons. Markedly larger transplants were present in 95% of 20 cases with E16 derived transplants. Sites in the ventral midbrain were successful, while limited or no survival occurred at sites more remote from IPN. Retrograde labeling of transplant neurons was present in each case studied with HRP injection into host IPN. Colloidal gold-labeled macrophages, some oriented capillaries and GFAP-positive processes marked the donor-host interface. In EM the interface was evident only by the difference in tissue elements in the transplant versus host. Numerous synapses of Gray types I and II were present in the transplant. Excellent survival of MH neurons, donor/host interfaces, innervation of IPN by the transplant and fine structure in and around the transplants, all suggest that such preparations are suitable for further experimental analysis of the habenulo-interpeduncular system. Hindawi Publishing Corporation 1989 /pmc/articles/PMC2565001/ /pubmed/2485117 http://dx.doi.org/10.1155/NP.1989.57 Text en Copyright © 1989.
spellingShingle Article
Lenn, Nicholas J.
Seeley, P. John
Field, Pauline M.
Raisman, Geoffrey
Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title_full Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title_fullStr Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title_full_unstemmed Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title_short Fetal Medial Habenula Transplants: Innervation of the Rat Interpeduncular Nucleus
title_sort fetal medial habenula transplants: innervation of the rat interpeduncular nucleus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565001/
https://www.ncbi.nlm.nih.gov/pubmed/2485117
http://dx.doi.org/10.1155/NP.1989.57
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