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Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results

The previous companion paper detailed, a technique which allowed embryonic retinal ganglion cell axons to grow from the anterior eye chamber across a PNS bridge, and enter the adult host forebrain. Embryonic eyes of E11, E14, E18 and E21 animals were sutured to a PNS bridge, the embryonic eye implan...

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Autores principales: Hallas, Brian H., Lowe, Howard F., Jacobsen, Glenn, LaCorte, Michael, Lee, Simone P., Zanakis, Michael F.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565008/
https://www.ncbi.nlm.nih.gov/pubmed/2485120
http://dx.doi.org/10.1155/NP.1989.87
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author Hallas, Brian H.
Lowe, Howard F.
Jacobsen, Glenn
LaCorte, Michael
Lee, Simone P.
Zanakis, Michael F.
author_facet Hallas, Brian H.
Lowe, Howard F.
Jacobsen, Glenn
LaCorte, Michael
Lee, Simone P.
Zanakis, Michael F.
author_sort Hallas, Brian H.
collection PubMed
description The previous companion paper detailed, a technique which allowed embryonic retinal ganglion cell axons to grow from the anterior eye chamber across a PNS bridge, and enter the adult host forebrain. Embryonic eyes of E11, E14, E18 and E21 animals were sutured to a PNS bridge, the embryonic eye implanted into an adult host eye, and the distal end of the bridge implanted into the host forebrain. Results indicate that when eyes of all ages are used for implantation, axons could be observed to grow from the embryonic retina, through the bridge and into the adult host forebrain. The axons extend for long distances in the host brain, reach various layers of the cortex and in a few animals enter the caudate/putamen complex. Control studies show that the bridge is used exclusively as the conduit to the brain, as opposed to the degenerated host optic nerve. Thus, the results presented in this paper indicate that successful grafting and transplantation is possible using the aforementioned technique. The results suggest that the described visual system reconstruction technique can be used for the study of development and transplantation in this system.
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spelling pubmed-25650082008-10-16 Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results Hallas, Brian H. Lowe, Howard F. Jacobsen, Glenn LaCorte, Michael Lee, Simone P. Zanakis, Michael F. J Neural Transplant Article The previous companion paper detailed, a technique which allowed embryonic retinal ganglion cell axons to grow from the anterior eye chamber across a PNS bridge, and enter the adult host forebrain. Embryonic eyes of E11, E14, E18 and E21 animals were sutured to a PNS bridge, the embryonic eye implanted into an adult host eye, and the distal end of the bridge implanted into the host forebrain. Results indicate that when eyes of all ages are used for implantation, axons could be observed to grow from the embryonic retina, through the bridge and into the adult host forebrain. The axons extend for long distances in the host brain, reach various layers of the cortex and in a few animals enter the caudate/putamen complex. Control studies show that the bridge is used exclusively as the conduit to the brain, as opposed to the degenerated host optic nerve. Thus, the results presented in this paper indicate that successful grafting and transplantation is possible using the aforementioned technique. The results suggest that the described visual system reconstruction technique can be used for the study of development and transplantation in this system. Hindawi Publishing Corporation 1989 /pmc/articles/PMC2565008/ /pubmed/2485120 http://dx.doi.org/10.1155/NP.1989.87 Text en Copyright © 1989.
spellingShingle Article
Hallas, Brian H.
Lowe, Howard F.
Jacobsen, Glenn
LaCorte, Michael
Lee, Simone P.
Zanakis, Michael F.
Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title_full Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title_fullStr Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title_full_unstemmed Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title_short Developing Retina and PNS Segments for Transplantation Into the Adult Host Eye: Reconstruction of the Mammalian Visual System. 2. Results
title_sort developing retina and pns segments for transplantation into the adult host eye: reconstruction of the mammalian visual system. 2. results
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565008/
https://www.ncbi.nlm.nih.gov/pubmed/2485120
http://dx.doi.org/10.1155/NP.1989.87
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