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Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences
The mechanism by which protein-coding portions of eukaryotic genes came to be separated by long non-coding stretches of DNA, and the purpose for this perplexing arrangement, have remained unresolved fundamental biological problems for three decades. We report here a plausible solution to this proble...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565106/ https://www.ncbi.nlm.nih.gov/pubmed/18941625 http://dx.doi.org/10.1371/journal.pone.0003456 |
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author | Regulapati, Rahul Bhasi, Ashwini Singh, Chandan Kumar Senapathy, Periannan |
author_facet | Regulapati, Rahul Bhasi, Ashwini Singh, Chandan Kumar Senapathy, Periannan |
author_sort | Regulapati, Rahul |
collection | PubMed |
description | The mechanism by which protein-coding portions of eukaryotic genes came to be separated by long non-coding stretches of DNA, and the purpose for this perplexing arrangement, have remained unresolved fundamental biological problems for three decades. We report here a plausible solution to this problem based on analysis of open reading frame (ORF) length constraints in the genomes of nine diverse species. If primordial nucleic acid sequences were random in sequence, functional proteins that are innately long would not be encoded due to the frequent occurrence of stop codons. The best possible way that a long protein-coding sequence could have been derived was by evolving a split-structure from the random DNA (or RNA) sequence. Results of the systematic analyses of nine complete genome sequences presented here suggests that perhaps the major underlying structural features of split-genes have evolved due to the indigenous occurrence of split protein-coding genes in primordial random nucleotide sequence. The results also suggest that intron-rich genes containing short exons may have been the original form of genes intrinsically occurring in random DNA, and that intron-poor genes containing long exons were perhaps derived from the original intron-rich genes. |
format | Text |
id | pubmed-2565106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-25651062008-10-20 Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences Regulapati, Rahul Bhasi, Ashwini Singh, Chandan Kumar Senapathy, Periannan PLoS One Research Article The mechanism by which protein-coding portions of eukaryotic genes came to be separated by long non-coding stretches of DNA, and the purpose for this perplexing arrangement, have remained unresolved fundamental biological problems for three decades. We report here a plausible solution to this problem based on analysis of open reading frame (ORF) length constraints in the genomes of nine diverse species. If primordial nucleic acid sequences were random in sequence, functional proteins that are innately long would not be encoded due to the frequent occurrence of stop codons. The best possible way that a long protein-coding sequence could have been derived was by evolving a split-structure from the random DNA (or RNA) sequence. Results of the systematic analyses of nine complete genome sequences presented here suggests that perhaps the major underlying structural features of split-genes have evolved due to the indigenous occurrence of split protein-coding genes in primordial random nucleotide sequence. The results also suggest that intron-rich genes containing short exons may have been the original form of genes intrinsically occurring in random DNA, and that intron-poor genes containing long exons were perhaps derived from the original intron-rich genes. Public Library of Science 2008-10-20 /pmc/articles/PMC2565106/ /pubmed/18941625 http://dx.doi.org/10.1371/journal.pone.0003456 Text en Senapathy et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Regulapati, Rahul Bhasi, Ashwini Singh, Chandan Kumar Senapathy, Periannan Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title | Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title_full | Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title_fullStr | Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title_full_unstemmed | Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title_short | Origination of the Split Structure of Spliceosomal Genes from Random Genetic Sequences |
title_sort | origination of the split structure of spliceosomal genes from random genetic sequences |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565106/ https://www.ncbi.nlm.nih.gov/pubmed/18941625 http://dx.doi.org/10.1371/journal.pone.0003456 |
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