Cargando…

Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing

We have previously described long-term survival of isolated bovine chromaffin cell suspension grafts in the periaqueductal gray of adult rats. Electron microscopic analysis of the graft sites revealed synapses on the transplanted chromaffin cells. The origin of these synapses is not known, but they...

Descripción completa

Detalles Bibliográficos
Autores principales: Ortega, John D., Sagen, Jacqueline, Pappas, George D.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565282/
https://www.ncbi.nlm.nih.gov/pubmed/7819371
http://dx.doi.org/10.1155/NP.1994.31
_version_ 1782159872725352448
author Ortega, John D.
Sagen, Jacqueline
Pappas, George D.
author_facet Ortega, John D.
Sagen, Jacqueline
Pappas, George D.
author_sort Ortega, John D.
collection PubMed
description We have previously described long-term survival of isolated bovine chromaffin cell suspension grafts in the periaqueductal gray of adult rats. Electron microscopic analysis of the graft sites revealed synapses on the transplanted chromaffin cells. The origin of these synapses is not known, but they are probably derived from the host since the initial grafts were suspensions of chromaffin cells that were essentially free of other cell types. In order to determine the origin of the observed synapses, retrograde and anterograde tracer analyses, were performed on grafted rats at 4 and 8 weeks after transplantation. Following injection of the retrograde tracer (Fluoro-Gold) into graft sites, four major host sites were labeled: hindbrain reticular formation, substantia nigra, lateral hypothalamus, and cingulate cortex. Injection of anterograde tracer (rhodamine-conjugated dextranamine) into the substantia nigra, lateral hypothalamus, and cingulate cortex produced labeled fibers and terminals in and around 4 and 8 week old chromaffin cell graft sites. An increase in both the number of retrogradely labeled cells, as well as in the density of anterogradely labeled fibers and terminals within the graft site, was observed from 4 to 8 weeks. This study shows that graft innervation from the host is primarily from areas that normally project afferent fibers to the periaqueductal gray. The increase in labeled fibers, and terminals over 8 weeks suggests that de novo synapse formation on grafted bovine chromaffin cells is a continuous process that is dependent on the regenerative capacity and plasticity of the host neuronal network and the grafted bovine chromaffin cells.
format Text
id pubmed-2565282
institution National Center for Biotechnology Information
language English
publishDate 1994
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-25652822008-10-16 Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing Ortega, John D. Sagen, Jacqueline Pappas, George D. J Neural Transplant Plast Article We have previously described long-term survival of isolated bovine chromaffin cell suspension grafts in the periaqueductal gray of adult rats. Electron microscopic analysis of the graft sites revealed synapses on the transplanted chromaffin cells. The origin of these synapses is not known, but they are probably derived from the host since the initial grafts were suspensions of chromaffin cells that were essentially free of other cell types. In order to determine the origin of the observed synapses, retrograde and anterograde tracer analyses, were performed on grafted rats at 4 and 8 weeks after transplantation. Following injection of the retrograde tracer (Fluoro-Gold) into graft sites, four major host sites were labeled: hindbrain reticular formation, substantia nigra, lateral hypothalamus, and cingulate cortex. Injection of anterograde tracer (rhodamine-conjugated dextranamine) into the substantia nigra, lateral hypothalamus, and cingulate cortex produced labeled fibers and terminals in and around 4 and 8 week old chromaffin cell graft sites. An increase in both the number of retrogradely labeled cells, as well as in the density of anterogradely labeled fibers and terminals within the graft site, was observed from 4 to 8 weeks. This study shows that graft innervation from the host is primarily from areas that normally project afferent fibers to the periaqueductal gray. The increase in labeled fibers, and terminals over 8 weeks suggests that de novo synapse formation on grafted bovine chromaffin cells is a continuous process that is dependent on the regenerative capacity and plasticity of the host neuronal network and the grafted bovine chromaffin cells. Hindawi Publishing Corporation 1994 /pmc/articles/PMC2565282/ /pubmed/7819371 http://dx.doi.org/10.1155/NP.1994.31 Text en Copyright © 1994 .
spellingShingle Article
Ortega, John D.
Sagen, Jacqueline
Pappas, George D.
Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title_full Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title_fullStr Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title_full_unstemmed Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title_short Origin of Afferent Projections into Bovine Chromaffin Cell Implants in the Rat Periaqueductal Gray Determined by Retrograde and Anterograde Tracing
title_sort origin of afferent projections into bovine chromaffin cell implants in the rat periaqueductal gray determined by retrograde and anterograde tracing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565282/
https://www.ncbi.nlm.nih.gov/pubmed/7819371
http://dx.doi.org/10.1155/NP.1994.31
work_keys_str_mv AT ortegajohnd originofafferentprojectionsintobovinechromaffincellimplantsintheratperiaqueductalgraydeterminedbyretrogradeandanterogradetracing
AT sagenjacqueline originofafferentprojectionsintobovinechromaffincellimplantsintheratperiaqueductalgraydeterminedbyretrogradeandanterogradetracing
AT pappasgeorged originofafferentprojectionsintobovinechromaffincellimplantsintheratperiaqueductalgraydeterminedbyretrogradeandanterogradetracing