Mutational optimization of the coelenterazine-dependent luciferase from Renilla

Renilla luciferase (RLUC) is a popular reporter enzyme for gene expression and biosensor applications, but it is an unstable enzyme whose catalytic mechanism remains to be elucidated. We titrated that one RLUC molecule can turn over about one hundred molecules of coelenterazine substrate. Mutagenesi...

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Detalles Bibliográficos
Autores principales: Woo, Jongchan, von Arnim, Albrecht G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565673/
https://www.ncbi.nlm.nih.gov/pubmed/18826616
http://dx.doi.org/10.1186/1746-4811-4-23

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565673/
https://www.ncbi.nlm.nih.gov/pubmed/18826616
http://dx.doi.org/10.1186/1746-4811-4-23

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