Cargando…
Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element
OBJECTIVES: Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566517/ https://www.ncbi.nlm.nih.gov/pubmed/18755696 http://dx.doi.org/10.1093/jac/dkn334 |
_version_ | 1782159944331558912 |
---|---|
author | Opintan, Japheth A. Newman, Mercy J. Nsiah-Poodoh, Owusu Agyemang Okeke, Iruka N. |
author_facet | Opintan, Japheth A. Newman, Mercy J. Nsiah-Poodoh, Owusu Agyemang Okeke, Iruka N. |
author_sort | Opintan, Japheth A. |
collection | PubMed |
description | OBJECTIVES: Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates. METHODS: Twenty-seven isolates from the outbreak were screened by PCR and sequencing for class 1 and 2 integrons and for the SXT element. RESULTS: Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron. CONCLUSIONS: Most strains characterized carried multiple elements conferring resistance to trimethoprim. This suggests that trimethoprim/sulfamethoxazole should not be used empirically in cholera treatment. |
format | Text |
id | pubmed-2566517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25665172009-02-25 Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element Opintan, Japheth A. Newman, Mercy J. Nsiah-Poodoh, Owusu Agyemang Okeke, Iruka N. J Antimicrob Chemother Original Research OBJECTIVES: Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates. METHODS: Twenty-seven isolates from the outbreak were screened by PCR and sequencing for class 1 and 2 integrons and for the SXT element. RESULTS: Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron. CONCLUSIONS: Most strains characterized carried multiple elements conferring resistance to trimethoprim. This suggests that trimethoprim/sulfamethoxazole should not be used empirically in cholera treatment. Oxford University Press 2008-11 2008-08-27 /pmc/articles/PMC2566517/ /pubmed/18755696 http://dx.doi.org/10.1093/jac/dkn334 Text en © The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org |
spellingShingle | Original Research Opintan, Japheth A. Newman, Mercy J. Nsiah-Poodoh, Owusu Agyemang Okeke, Iruka N. Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title | Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title_full | Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title_fullStr | Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title_full_unstemmed | Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title_short | Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element |
title_sort | vibrio cholerae o1 from accra, ghana carrying a class 2 integron and the sxt element |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566517/ https://www.ncbi.nlm.nih.gov/pubmed/18755696 http://dx.doi.org/10.1093/jac/dkn334 |
work_keys_str_mv | AT opintanjaphetha vibriocholeraeo1fromaccraghanacarryingaclass2integronandthesxtelement AT newmanmercyj vibriocholeraeo1fromaccraghanacarryingaclass2integronandthesxtelement AT nsiahpoodohowusuagyemang vibriocholeraeo1fromaccraghanacarryingaclass2integronandthesxtelement AT okekeirukan vibriocholeraeo1fromaccraghanacarryingaclass2integronandthesxtelement |