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An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase–DNA complexes to surfaces
Single molecule analysis of individual enzymes can require oriented immobilization of the subject molecules on a detection surface. As part of a technology development project for single molecule DNA sequencing, we faced the multiple challenges of immobilizing both a DNA polymerase and its DNA templ...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566871/ https://www.ncbi.nlm.nih.gov/pubmed/18723573 http://dx.doi.org/10.1093/nar/gkn531 |